IGEM:IMPERIAL/2007/Projects/Cell by date/Modelling: Difference between revisions
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==Overview of Modelling == | ==Overview of Modelling == | ||
Welcome to our portal page for the modelling of Cell By Date | |||
Cell by date tries to improve on printed cell by date as acting as a thermal exposure device, exploiting the thermal dependance of the rate of expression of a simple reporter system. We are looking at a variety of constructs to realise this behaviour. | |||
===Constructs to be Used === | |||
{| | |||
|- | |||
|GFP Based | |||
|[[IMAGE:ICGEMS_dvc_Ptet_gfp.png|left|frame]] | |||
|[[IMAGE:ICGEMS_dvc_PT7_gfp.png|left|frame]] | |||
|[[IMAGE:PcI_construct.PNG|left|frame]] | |||
|- | |||
|RFP Based | |||
|[[IMAGE:ICGEMS_dvc_Ptet_rfp.PNG|left|frame]] | |||
|[[IMAGE:ICGEMS_dvc_PT7_rfp.PNG|left|frame]] | |||
|[[IMAGE:ICGEMS_dvc_PcI_rfp.PNG|left|frame]] | |||
|- | |||
|} | |||
As can be seen in the above table all of our constructs have the same general form of a promoter upstream of a reporter. Looking at the rate of Fluorescent Protein (FP) produced : | |||
<math>\frac{d[FP]}{dt}=k_{FP}(t)-d_{FP}[FP] </math> | |||
# kFP : Function of Temperature. k is based on the promoter used as promoters take time to turn on. | |||
# dFP : Function of System. May be considered to be a function of temperature as proteins degrade faster at higher temperatures. | |||
{| cellspacing="5" | |||
|-valign="top" | |||
|width=50%| | |||
<!--ROW 2--> | |||
|-valign="top" | |||
|| | |||
===Transient Response of System=== | |||
Image needs to be changed to matlab plot of system | |||
[[Image:ConstantTempCBDresponseC.png|left|500px]]<br clear = "all"> | |||
|| | |||
=== | ===Transfer Function of System=== | ||
Image needs to be changed to matlab plot of system | |||
[[Image: CellByDate_tf.jpg|thumb|left|500px|Construct 2 - LuxR not expressed]]<br clear = "all"> | |||
|} | |||
== | ===Feedback from Protocols/"Manufacturing"=== | ||
=== | ====Construct to be pursued==== | ||
*pTet | |||
Refer to the [[IGEM:IMPERIAL/2007/DNA_Constructs|constructs]] page for more information. | |||
=== | ===Performance Specifications=== | ||
Latest revision as of 08:56, 30 August 2007
Cell by Date: Modelling
Overview of Modelling
Welcome to our portal page for the modelling of Cell By Date
Cell by date tries to improve on printed cell by date as acting as a thermal exposure device, exploiting the thermal dependance of the rate of expression of a simple reporter system. We are looking at a variety of constructs to realise this behaviour.
Constructs to be Used
GFP Based | |||
RFP Based |
As can be seen in the above table all of our constructs have the same general form of a promoter upstream of a reporter. Looking at the rate of Fluorescent Protein (FP) produced :
[math]\displaystyle{ \frac{d[FP]}{dt}=k_{FP}(t)-d_{FP}[FP] }[/math]
- kFP : Function of Temperature. k is based on the promoter used as promoters take time to turn on.
- dFP : Function of System. May be considered to be a function of temperature as proteins degrade faster at higher temperatures.
| |
Transient Response of SystemImage needs to be changed to matlab plot of system |
Transfer Function of SystemImage needs to be changed to matlab plot of system |
Feedback from Protocols/"Manufacturing"
Construct to be pursued
- pTet
Refer to the constructs page for more information.