IGEM:IMPERIAL/2007/Projects/Cell by date/Modelling: Difference between revisions
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===Feedback from Protocols/"Manufacturing"=== | ===Feedback from Protocols/"Manufacturing"=== | ||
==== | ====Construct to be pursued==== | ||
*pTet | *pTet | ||
Latest revision as of 08:56, 30 August 2007
Cell by Date: Modelling
Overview of Modelling
Welcome to our portal page for the modelling of Cell By Date
Cell by date tries to improve on printed cell by date as acting as a thermal exposure device, exploiting the thermal dependance of the rate of expression of a simple reporter system. We are looking at a variety of constructs to realise this behaviour.
Constructs to be Used
GFP Based | |||
RFP Based |
As can be seen in the above table all of our constructs have the same general form of a promoter upstream of a reporter. Looking at the rate of Fluorescent Protein (FP) produced :
[math]\displaystyle{ \frac{d[FP]}{dt}=k_{FP}(t)-d_{FP}[FP] }[/math]
- kFP : Function of Temperature. k is based on the promoter used as promoters take time to turn on.
- dFP : Function of System. May be considered to be a function of temperature as proteins degrade faster at higher temperatures.
| |
Transient Response of SystemImage needs to be changed to matlab plot of system |
Transfer Function of SystemImage needs to be changed to matlab plot of system |
Feedback from Protocols/"Manufacturing"
Construct to be pursued
- pTet
Refer to the constructs page for more information.