IGEM:IMPERIAL/2007/Projects/Experimental Design
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We have identified three phases of experiments for each of the project:
- Phase 1 - Intial testing of the promoters and test constructs in vitro
- Phase 2 - Testing/Validation of the test constructs
- Phase 3 - Testing/Validation of final constructs
In addition any overlap between the experiments of the projects has been identified and considered when making the experimental design.
Time table
Testing of constructs and cell extracts
Week 8 | Week 9 | Week 10 | |
Monday | - | Refine the wiki | |
Tuesday | Prepare GFP solution-calibration Construct Calibration Curve Refine the wiki |
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Wednesday | Test Home made cell extract Test varying levels of cell extract |
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Thursday | |||
Friday | Prepare GFP solution-calibration Construct Calibration Curve |
CBD
Week 8 | Week 9 | Week 10 | |
Monday | - | Exp with 10 and 45 °C, continue measuring 30 and 40 °C | |
Tuesday | Phase 1 - test pT7 | Exp with 15 and 25 °C | |
Wednesday | Exp with 30 and 40 °C, continue measuring 15 and 25 °C | ||
Thursday | Test 20 and 37 °C, Test GFP degradation at 20 and 37 °C |
Test suitable gradient of temperature, minimum working temp - maximum working temp | |
Friday | Second day testing 20 and 37 °C | Test suitable step increases |
ID
Week 8 | Week 9 | Week 10 | |
Monday | - | ||
Tuesday | test pLux in-vitro - 1nM AHL | Refine AHL concentrations range 25°C Purify LuxR if plasmid arrives |
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Wednesday | Choose a range of AHL concentrations range 37°C Purify LuxR if plasmid arrives |
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Thursday | Test purified LuxR | ||
Friday | Test construct with 5 AHL concentrations - 25 °C | Test purified LuxR |