IGEM:IMPERIAL/2007/Projects/Experimental Design
From OpenWetWare
We have identified three phases of experiments for each of the project:
- Phase 1 - Intial testing of the promoters and test constructs in vitro
- Phase 2 - Testing/Validation of the test constructs
- Phase 3 - Testing/Validation of final constructs
In addition any overlap between the experiments of the projects has been identified and considered when making the experimental design.
Problems page
- Contains a list of all the problems being faced by the team regarding the experiments being carried out
Time table
Testing of constructs and cell extracts
| Week 8 | Week 9 | Week 10 | |
| Monday | - | Refine the wiki | |
| Tuesday | Prepare GFP solution-calibration Construct Calibration Curve Refine the wiki |
||
| Wednesday | Test Home made cell extract Test varying levels of cell extract |
||
| Thursday | |||
| Friday | Prepare GFP solution-calibration Construct Calibration Curve |
CBD
| Week 8 | Week 9 | Week 10 | |
| Monday | - | Exp with 15 and 25 °C | |
| Tuesday | Phase 1 - test pT7 | Exp with 30 and 40 °C, continue measuring 15 and 25 °C | |
| Wednesday | Exp with 10 and 45 °C, continue measuring 30 and 40 °C | ||
| Thursday | Test 20 and 37 °C, Test GFP degradation at 20 and 37 °C |
Test suitable step increases | |
| Friday | Second day testing 20 and 37 °C | Test suitable gradient of temperature, minimum working temp - maximum working temp |
ID
| Week 8 | Week 9 | Week 10 | |
| Monday | - | ||
| Tuesday | test pLux in-vitro - 1nM AHL | Refine AHL concentrations range 25°C Purify LuxR if plasmid arrives |
|
| Wednesday | Choose a range of AHL concentrations range 37°C Purify LuxR if plasmid arrives |
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| Thursday | Test purified LuxR | ||
| Friday | Test construct with 5 AHL concentrations - 25 °C | Test purified LuxR |
