IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes: Difference between revisions
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|| Home-made cell extracts (by virtue of its large quantity) must be used|| Fluctuation of temperature | || Home-made cell extracts (by virtue of its large quantity) must be used|| Fluctuation of temperature | ||
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|| Home made cell extracts might not be reliable|| Only 50µL is used per well, and this amount is less than the optimum amount for measurement by the fluorometer | |||
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Revision as of 18:11, 13 August 2007
Batch Mode | Plates | ||||
Pros | Minimises evaporation | Less samples used | |||
No temperature fluctuations | No pipetting involved from stock solution | ||||
Cons | Alot of cell extract is needed | Problem with evaporation | |||
Home-made cell extracts (by virtue of its large quantity) must be used | Fluctuation of temperature | Home made cell extracts might not be reliable | Only 50µL is used per well, and this amount is less than the optimum amount for measurement by the fluorometer |