IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Peixuan Pey (talk | contribs) No edit summary |
Peixuan Pey (talk | contribs) No edit summary |
||
| Line 31: | Line 31: | ||
| style="background:#aabadd" | | | style="background:#aabadd" | | ||
|| Home-made cell extracts (by virtue of its large quantity) must be used|| Fluctuation of temperature | || Home-made cell extracts (by virtue of its large quantity) must be used|| Fluctuation of temperature | ||
|- style="background:#d5dcef" | |||
| style="background:#aabadd" | | | style="background:#aabadd" | | ||
|| Home made cell extracts might not be reliable|| Only 50µL is used per well, and this amount is less than the optimum amount for measurement by the fluorometer | || Home made cell extracts might not be reliable|| Only 50µL is used per well, and this amount is less than the optimum amount for measurement by the fluorometer | ||
|} | |} | ||
Revision as of 18:11, 13 August 2007
| Batch Mode | Plates | |
| Pros | Minimises evaporation | Less samples used |
| No temperature fluctuations | No pipetting involved from stock solution | |
| Cons | Alot of cell extract is needed | Problem with evaporation |
| Home-made cell extracts (by virtue of its large quantity) must be used | Fluctuation of temperature | |
| Home made cell extracts might not be reliable | Only 50µL is used per well, and this amount is less than the optimum amount for measurement by the fluorometer |
