IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes: Difference between revisions
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===Comparison of Batch mode and Plate mode=== | |||
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Revision as of 03:35, 14 August 2007
Comparison of Batch mode and Plate mode
| Batch Mode | Plates | |
| Pros | Minimises evaporation | Less samples used |
| No temperature fluctuations | No pipetting involved from stock solution | |
| Cons | Alot of cell extract is needed | Problem with evaporation |
| Home-made cell extracts (by virtue of its large quantity) must be used | Fluctuation of temperature | |
| Home made cell extracts might not be reliable | Only 50µL is used per well, and this amount is less than the optimum amount for measurement by the fluorometer |
