IGEM:IMPERIAL/2007/Projects/In-Veso/Notes: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 26: Line 26:
<br>'''Thur''': Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract
<br>'''Thur''': Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract
<br>'''Fri''': Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract
<br>'''Fri''': Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract


===Week 5===
===Week 5===

Revision as of 15:26, 29 July 2007

In-Veso Gene Expression: Notes



Timeline

Week 4


Mon: Order reagents
Tue: -
Wed: Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract
Thur: Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract
Fri: Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract


Week 5


Mon: Test if S30 or S12 is better for the respective strains of E.coli
Tue: Carry out temperature and pH tests on in vitro and in veso extracts
Wed: Carry out temperature and pH tests on in vitro and in veso extracts
Thur: Carry out temperature and pH tests on in vitro and in veso extracts
Fri: Carry out temperature and pH tests on in vitro and in veso extracts