IGEM:IMPERIAL/2007/Projects/In-Veso/Presentations/13-09-07: Difference between revisions

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The news this week is that we now have vesicles expressing GFP.
The news this week is that we now have vesicles '''''with GENE EXPRESSION'''''.
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Revision as of 15:26, 12 September 2007


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Jamboree


Winning


Chassis




Vitro v Vivo

Non-pathogenic Pathogenic
Quick and Simple Laborious and Time Consuming
Good Control Poor Control
Circular and Linear DNA Circular DNA Only
Minimal Protein Degradation Endogenous Proteases
Can synthesise toxic proteins Toxic proteins kill the cells
Short lifespan Long lifespan
Expensive Cheap
Less characterized, not so popular Well characterized, popular


Yeah, but In Vitro has its own problems.

Less characterized, not so popular Well, that's what we are trying to change.
Expensive Can't do much about this...
Short lifespan Golly gee, what can we do about that?


The New Chassis: In Veso Gene Expression

Vesicles can be made to last for days, weeks, months...


But how is this Synthetic Biology?

    True Biological Engineering
    Minimal, Elegant
    Functional
    Specific
    Applications, Applications
    Drug delivery
    Research
    Artificial Cells
    Tissue Engineering
    Biofilm detection...
    Vesicles can make Synthetic Biology useful in the Real WorldTM


Right then, tell me about them vesicles.

As we saw last week, we can make vesicles.

    The news this week is that we now have vesicles with GENE EXPRESSION.


Characterisation



But what about the applications?



So how do we present at the Jamboree?



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