IGEM:IMPERIAL/2007/Projects/In-Veso/Specifications: Difference between revisions
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| || PoPS Effect || Lifespan || Protocol || Membrane Traffic || Waste | | || PoPS Effect || Lifespan || Protocol || Membrane Traffic || Waste | ||
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| style="background:#aabadd" | [[#Temperature]] | | style="background:#aabadd" | [[#Temperature|Temperature]] | ||
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| style="background:#aabadd" | [[#Media]] | | style="background:#aabadd" | [[#Media|Media]] | ||
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| style="background:#aabadd" | [[#pH]] | | style="background:#aabadd" | [[#pH|pH]] | ||
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| style="background:#aabadd" | [[#Piping]] | | style="background:#aabadd" | [[#Piping|Piping]] | ||
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| style="background:#aabadd" | [[#Centrifuge]] | | style="background:#aabadd" | [[#Centrifuge|Centrifuge]] | ||
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Revision as of 07:38, 26 July 2007
In-Veso Gene Expression: Specifications
Characterization
Characterisation Chart
| PoPS Effect | Lifespan | Protocol | Membrane Traffic | Waste | |
| Temperature | |||||
| Media | |||||
| pH | |||||
| Piping | |||||
| Centrifuge |
Temperature
Range from 0-40oC
Media
- Water
- Agar Gel
- Polyacrylamide
- Paper
- E. coli Biofilm
pH
Range from 6.0 to 8.0
Piping
How narrow a pipe, and how fast, can the vesicles be passed through without bursting? Testing range..?
This is valuable for determining the spray system to be used in the Biofilm application. It also helps with laboratory manipulations - pipetting, etc.
Centrifuge
How fast can the vesicles be centrifuged without destruction? Testing range...?
Protocol
Rate of PoPs
Comparison with the efficiency of other chassis
Limitations of type of proteins made in the vesicles
Life span
Membrane Permeability
Physical Conditions
temperature, pH, media, centrifugation
