IGEM:IMPERIAL/2007/Projects/In-Veso/Specifications: Difference between revisions
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The specifications outline the characteristics of the chassis being sought, and the quantities that will be varied in experiments. The range for each input variable is also detailed. All lists are prioritised in order to help planning of experiments. | |||
== | == Input Variables == | ||
'''Temperature''' | |||
Range from 4-37<sup>o</sup>C with priorities at: | |||
# 25<sup>o</sup>C | |||
# 4<sup>o</sup>C | |||
# 6-20<sup>o</sup>C | |||
# 30-37<sup>o</sup>C | |||
'''Media''' | |||
These are the different media the technique should be tested in, in order of priority: | These are the different media the technique should be tested in, in order of priority: | ||
# Water | # Water | ||
# X-Gal | |||
# E. coli Biofilm | |||
# Agar Gel | # Agar Gel | ||
# Polyacrylamide | # Polyacrylamide | ||
# Paper | # Paper | ||
Note: take into account Osmolarity of the media. | |||
'''Parts''' | |||
List of parts from the registry we would like to test in-veso. | |||
'''Piping''' | |||
How narrow a pipe, and how fast, can the vesicles be passed through without bursting? Testing range..? | How narrow a pipe, and how fast, can the vesicles be passed through without bursting? Testing range..? | ||
This is valuable for determining the spray system to be used in the Biofilm application. It also helps with laboratory manipulations - pipetting, etc. | This is valuable for determining the spray system to be used in the Biofilm application. It also helps with laboratory manipulations - pipetting, etc. | ||
==== | == Dependent Quantities == | ||
'''Life span''' | |||
* Measured in hours | |||
'''Rate of protein synthesis''' | |||
* The units will depend on the reporter being used, as well as the volumetric unit. | |||
'''Rate of PoPs''' | |||
* Indirectly measured in GFP synthesis per CFU per second. | |||
'''Membrane Traffic''' | |||
* List of particles that can cross the α-hemolysin pores | |||
== Resources == | |||
These are the resources that are available to us: | |||
* IC Specialists | |||
* [[IGEM:IMPERIAL/2007/Projects/In-Veso/References|Literature]] | |||
* Wetlab | |||
== Characterisation Chart == | |||
The following chart cross-references the input variables to the dependent quantities. Each cell will link to the protocols in the design section relevant to that relationship. | |||
{| style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" | |||
|- style="background:#aabadd; color:Black" | |||
| || Lifespan || Rate of Protein <br> Synthesis || PoPS Effect || Membrane Traffic | |||
|- style="background:#d5dcef" | |||
| style="background:#aabadd" | [[#Temperature|Temperature]] | |||
|| [[IGEM:IMPERIAL/2007/Projects/In-Veso/Design/Protocols#Lifespan as a function of Temperature | 1]] || [[IGEM:IMPERIAL/2007/Projects/In-Veso/Design/Protocols#Protein Synthesis as a function of Temperature | 3]] || || | |||
|- style="background:#d5dcef" | |||
| style="background:#aabadd" | [[#Media|Media]] | |||
|| [[IGEM:IMPERIAL/2007/Projects/In-Veso/Design/Protocols#Lifespan as a function of Media | 2]] || [[IGEM:IMPERIAL/2007/Projects/In-Veso/Design/Protocols#Protein Synthesis as a function of Media | 4]] || || | |||
|- style="background:#d5dcef" | |||
| style="background:#aabadd" | [[#Parts|Parts]] | |||
|| || || || | |||
|- style="background:#d5dcef" | |||
| style="background:#aabadd" | [[#Piping|Piping]] | |||
|| || || || | |||
|} | |||
== | == Things to look out for == | ||
* Comparison with the efficiency of other chassis | |||
* Limitations of type of proteins made in the vesicles | |||
Latest revision as of 02:41, 27 July 2007
In-Veso Gene Expression: Specifications
The specifications outline the characteristics of the chassis being sought, and the quantities that will be varied in experiments. The range for each input variable is also detailed. All lists are prioritised in order to help planning of experiments.
Input Variables
Temperature
Range from 4-37oC with priorities at:
- 25oC
- 4oC
- 6-20oC
- 30-37oC
Media
These are the different media the technique should be tested in, in order of priority:
- Water
- X-Gal
- E. coli Biofilm
- Agar Gel
- Polyacrylamide
- Paper
Note: take into account Osmolarity of the media.
Parts
List of parts from the registry we would like to test in-veso.
Piping
How narrow a pipe, and how fast, can the vesicles be passed through without bursting? Testing range..?
This is valuable for determining the spray system to be used in the Biofilm application. It also helps with laboratory manipulations - pipetting, etc.
Dependent Quantities
Life span
- Measured in hours
Rate of protein synthesis
- The units will depend on the reporter being used, as well as the volumetric unit.
Rate of PoPs
- Indirectly measured in GFP synthesis per CFU per second.
Membrane Traffic
- List of particles that can cross the α-hemolysin pores
Resources
These are the resources that are available to us:
- IC Specialists
- Literature
- Wetlab
Characterisation Chart
The following chart cross-references the input variables to the dependent quantities. Each cell will link to the protocols in the design section relevant to that relationship.
| Lifespan | Rate of Protein Synthesis |
PoPS Effect | Membrane Traffic | |
| Temperature | 1 | 3 | ||
| Media | 2 | 4 | ||
| Parts | ||||
| Piping |
Things to look out for
- Comparison with the efficiency of other chassis
- Limitations of type of proteins made in the vesicles
