IGEM:IMPERIAL/2007/Projects/In-Veso/Specifications

In-Veso Gene Expression: Specifications

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

Characterization

Characterisation Chart

	PoPS Effect	Lifespan	Protocol	Membrane Traffic	Waste
Temperature
Media
pH
Piping
Centrifuge

Temperature

Range from 0-40^oC

Media

These are the different media the technique should be tested in, in order of priority:

1. Water
2. Agar Gel
3. β-Galactosidase
4. Polyacrylamide
5. Paper
6. E. coli Biofilm

Tom 10:56, 26 July 2007 (EDT) Just a note to take into account Osmolarity of the media, which I'm sure you've thought of already!

pH

Range from 6.0 to 8.0

Piping

How narrow a pipe, and how fast, can the vesicles be passed through without bursting? Testing range..?

This is valuable for determining the spray system to be used in the Biofilm application. It also helps with laboratory manipulations - pipetting, etc.

Centrifuge

How fast can the vesicles be centrifuged without destruction? Testing range...?

Physical Conditions

temperature, pH, media, centrifugation