IGEM:IMPERIAL/2007/Projects/In-Veso/Specifications

In-Veso Gene Expression: Specifications

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

Characterization

Resources

• IC Specialists
• Literature
• Wetlab

Characterisation Chart

	Lifespan	Rate of Protein Synthesis	PoPS Effect	Membrane Traffic	Waste
Temperature
Media
Piping
Parts

Temperature

Range from 4-37^oC with priorities at:

1. 25^oC
2. 4^oC
3. 6-20^oC
4. 30-37^oC

Media

These are the different media the technique should be tested in, in order of priority:

1. Water
2. X-Gal
3. E. coli Biofilm
4. Agar Gel
5. Polyacrylamide
6. Paper

Tom 10:56, 26 July 2007 (EDT) Just a note to take into account Osmolarity of the media, which I'm sure you've thought of already! Also, B-Galactosidase is an enzyme not a type of media?

Piping

How narrow a pipe, and how fast, can the vesicles be passed through without bursting? Testing range..?

This is valuable for determining the spray system to be used in the Biofilm application. It also helps with laboratory manipulations - pipetting, etc.

Parts

List of parts from the registry we would like to test in-veso.