IGEM:IMPERIAL/2007/Projects/Shopping Lists: Difference between revisions

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__NOTOC__
<!-- Heading -->
{| style="width:800px; height:80px;" cellpadding="0" border="0"
|-
| width="220px" | <html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html>
| width="390px" |  <br><center><html><h3>THE IMPERIAL iGEM 2007 Team </h3></html></center>
| width="110px"| <html><a href="http://www.imperial.ac.uk/centenary"><p align="right"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/7813700.GIF" height="60"></p></a></html>
|}
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{| style="width:800px;" cellpadding="2" cellspacing="0" align="center"
|- style="background:#000000; color:white;" align="center"
| [[IGEM:IMPERIAL/2007|<font face="Arial" size=1 style="color:#ffffff"> '''Home'''</font>]]
| [[IGEM:IMPERIAL/2007/Projects/Cell By Date|<font face="Arial" size=1 style="color:#ffffff"> '''Cell By Date'''</font>]]
| [[IGEM:IMPERIAL/2007/Biofilm Detector|<font face="Arial" size=1 style="color:#ffffff"> '''Infector Detector'''</font>]]
| [[IGEM:IMPERIAL/2007/Wet Lab|<font face="Arial" size=1 style="color:#ffffff"> '''Wet Lab'''</font>]]
| [[IGEM:IMPERIAL/2007/Dry Lab|<font face="Arial" size=1 style="color:#ffffff"> '''Dry Lab'''</font>]]
| [[IGEM:IMPERIAL/2007/Resources|<font face="Arial" size=1 style="color:#ffffff">'''Resources'''</font>]]
| [[IGEM:IMPERIAL/2007/Meet The Team|<font face="Arial" size=1 style="color:#ffffff"> '''Meet the
Team'''</font>]]
| [[IGEM:IMPERIAL/2007/Entertainment|<font face="Arial" size=1 style="color:#ffffff"> '''Entertainment'''</font>]]
| [[IGEM:IMPERIAL/2007/Acknowledgments|<font face="Arial" size=1 style="color:#ffffff"> '''Acknowledgments'''</font>]]
|}
<br>
{| cellpadding="0" border="0" align="center"
|width="200px" class="white" valign="top"|
{|class="white" align="left" cellpadding="0"
|- valign="center"
| {{Click || image=Service_course.png | link=IGEM:IMPERIAL/2007/Wet Lab/Lab Notebook| width=40px | height=40px }}
| [[IGEM:IMPERIAL/2007/Wet Lab/Lab Notebook|<font face="Arial" size=2 style="color:#000099">'''Lab Notebook'''</font>]]<br /> <font size=1>Daily Record of Wet Lab</font>
|-
| <br>
|- valign="center"
| {{Click || image=Service_protocols.png | link=IGEM:IMPERIAL/2007/Wet Lab/Protocols| width=40px | height=40px }}
| [[IGEM:IMPERIAL/2007/Wet Lab/Protocols|<font face="Arial" size=2 style="color:#000099">'''Protocols'''</font>]]<br /> <font size=1>Fabrication & Testing Protocols</font>
|-
| <br>
|- valign="center"
| {{Click || image=Service_notebook.png | link=IGEM:IMPERIAL/2007/Wet Lab/DNA Constructs| width=40px | height=40px }}
| [[IGEM:IMPERIAL/2007/Wet Lab/DNA Constructs|<font face="Arial" size=2 style="color:#000099">'''DNA Constructs'''</font>]]<br /> <font size=1>Overview of DNA Constructs</font>
|-
| <br>
|- valign="center"
| {{Click || image=Scientificwriters.png | link=IGEM:IMPERIAL/2007/Wet Lab/Shopping List| width=40px | height=40px }}
| [[IGEM:IMPERIAL/2007/Wet Lab/Shopping List|<font face="Arial" size=2 style="color:#000099">'''Shopping List'''</font>]]<br /> <font size=1>Expenditures on Materials</font>
|}
<br clear="all">
|width="560px" class="white" valign="top" |
==Shopping List==
==Shopping List==
{| style="background:#F5FAFF; border: 1px solid #aabadd; color:Black"
{| style="background:#F5FAFF; border: 1px solid #aabadd; color:Black"
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|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#19|19]]
| style="background:#aabadd" | [[#19|19]]
|| 09/08||  || || ||
|| 09/08|| L-Phosphatidic acid sodium from Egg Yolk || 2042549 || 1 || 10.92
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#20|20]]
| style="background:#aabadd" | [[#20|20]]
|| 09/08|| || || ||
|| 09/08|| Mineral Oil Molecular Biology Reagent || 2042549 || 500ml || 18.35
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#21|21]]
| style="background:#aabadd" | [[#21|21]]
||09/08 || ||  || ||
||09/08 || 1,2-Dioleoyl-Sn-glycero-3-phosphocholine ||  2042549|| 5ml || 53.70
||- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#22|22]]
| style="background:#aabadd" | [[#22|22]]
|| 09/08|| || || ||
|| 09/08|| L-Amino Acids Kit || 2042549 || 1 || 203
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#23|23]]
| style="background:#aabadd" | [[#23|23]]
|| 09/08|| ||  || ||
|| 09/08|| T7 RNA Polymerase Recombinant; Expressed ||  2042549|| 10mg/ml || 29.9
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#24|24]]
| style="background:#aabadd" | [[#24|24]]
|| 09/08|| || || ||
|| 09/08|| Adenosine 5-Triphosphate Standard *Disodium || 2042549 || 5vl || 63.82
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#25|25]]
| style="background:#aabadd" | [[#25|25]]
|| 09/08|| || || ||
|| 09/08|| Phospho(enol)pyruvis acid monopotassium || 2042549 || 1g || 141.07
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#26|26]]
| style="background:#aabadd" | [[#26|26]]
|| 09/08|| || || ||
|| 09/08|| Pyruvate Kinase Tpe VIII from Bacillus || 2042549 || 1 || 135.51
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#27|27]]
| style="background:#aabadd" | [[#27|27]]
|| 09/08|| || || ||
|| 09/08|| Isopropyl B-D-Thiogalactopyranoside *Dioxane || 2042549 || 5g || 121.50
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#28|28]]
| style="background:#aabadd" | [[#28|28]]
|| 09/08|| ||  || ||
|| 09/08|| Magnesium Acetate Solution, for Molecular Biology ||  2042549|| 500ml || 120
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#29|29]]
| style="background:#aabadd" | [[#29|29]]
|| 09/08|| || || ||
|| 09/08|| L-Glutamic Acid Monopotassium || 2042549 || 500g || 66.88
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#30|30]]
| style="background:#aabadd" | [[#30|30]]
|| 09/08||  ||  ||  ||
|| 09/08|| YT Microbial Medium || 2042549 || 250g || 22.70 || 1888.62
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#31|31]]
|| 09/08|| Geneart ||  ||  || 125.09 ||2013.71
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#32|32]]
|| 14/08|| E.coli S30 Extract System for Circular DNA (Promega)|| L1020 || 30 reactions || 139
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#33|33]]
|| 14/08|| E.coli T7 S30 Extract System for Circular DNA (Promega)|| L1130 || 30 reactions || 139
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#34|34]]
|| 14/08|| Nuclease free water (Promega) || - || 2 x 25µL || 21
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#35|35]]
|| 14/08|| Delivery charges || - || - || 15 || 2202.62
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#36|36]]
|| 14/08|| Red Filter || - || 1 || 140
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#37|37]]
|| 14/08|| Red Filter || - || 1 || 198
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#38|38]]
|| 14/08|| Delivery charges || - || - || 10 || 2550.62
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#39|39]]
||17/08 || Oligos(MWG) || - || - || 15.64
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#40|40]]
||17/08 || Sealing mats(Fischer) || - || - || 16.00
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#41|41]]
||21/08||ATP||A7699||1g||38.60
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#42|42]]
||21/08||CTP||30320||100mg||34.70
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#43|43]]
||21/08||GTP||G8877||25mg||17.70
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#44|44]]
||21/08||UTP||94370||250mg||23.70
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#45|45]]
||21/08||E.coli tRNA||R4251||500UN||52.60
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#46|46]]
||21/08||cAMP||01895||100mg||13.40
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#47|47]]
||21/08||Folinic acid||F8259||25mg||21.00||2783.96
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#2|2]]
| style="background:#aabadd" | [[#48|48]]
|| 09/08|| || || ||
||28/8||Recombinant GFP (Clontech)||QV071299|| 1 UN|| 221.25 (25% discount)
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#49|49]]
||28/8||Recombinant DsRED Express (Clontech)||QV071299||1 UN||224.25 (25% discount)
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#50|50]]
||28/8||Delivery for Clontech ||- || -|| 30
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#51|51]]
|| 28/8||Qiagen Maxi Kit || Cat no.12162||1 ||107.10
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#52|52]]
||28/8 ||Delivery Charges || ||- ||12.00
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#53|53]]
||28/8 ||L-A-Phosphatidyl Choline B Palmitoyl-*GA ||- || 1 UN||140.50
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#54|54]]
||28/8 ||Sorbitan Monooleate ||- || 1 UN||10.08
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#55|55]]
|| 28/8||Dodecane. Anhydrous, 99+ || -||1 UN ||28.60||3557.74
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#56|56]]
|| 29/8||E.coli S30 Extract System for Circular DNA (Promega) || L1020||1 UN ||139.00
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#57|57]]
|| 29/8|| Delivery charges||- ||- || 15||3711.74
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#58|58]]
|| 03/09||E.coli S30 Extract System for Circular DNA (Promega) ||L1020 || 3 UN || 417.00
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#59|59]]
|| 03/09||Delivery Charges ||- ||- ||15
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#60|60]]
|| 03/09||Dodecane || -||500ml || 84.00
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#61|61]]
|| 03/09||Delivery ||- ||- ||13.00 || 4240.74
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#2|2]]
| style="background:#aabadd" | [[#62|62]]
|| 09/08|| || || ||
|| 04/10||Sequencing of DNA constructs||- ||- ||170.00
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | [[#2|2]]
| style="background:#aabadd" | [[#63|63]]
|| 09/08|| || || ||
||04/10 ||E.coli S30 Extract System for Circular DNA (Promega) || L1020||2 UN ||278.00
|}
 
==Acknowledgements==
#Geneart is giving us 10000 euros worth of products.
#Promega gave us a 50% discount for the all of the E.coli S30 extract system and E.coli T7 S30 extract system for circular DNA we have purchsed.
#Clontech is giving us 25% discount for all our purchases made.
 
|}

Latest revision as of 18:50, 4 October 2007

<html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html>
<html><h3>THE IMPERIAL iGEM 2007 Team </h3></html>
 <html><a href="http://www.imperial.ac.uk/centenary"><p align="right"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/7813700.GIF" height="60"></p></a></html>
Home Cell By Date Infector Detector Wet Lab Dry Lab Resources Meet the Team Entertainment Acknowledgments


Lab Notebook
Daily Record of Wet Lab

Protocols
Fabrication & Testing Protocols

DNA Constructs
Overview of DNA Constructs

Shopping List
Expenditures on Materials


Shopping List

Date Item Order No. Quantity Price Total Cost
1 01/08 Shipping 2039605 17
2 01/08 HiSpeed Plasmid Midi Kit 2039605 25 157.25
3 01/08 QIAquick Gel Extractino Kit 2039605 50 58.65
4 01/08 QIAprep Spin Miniprep Kit 2039605 50 47.85
5 01/08 Kanamycin Monosulfate from Streptomyces 2039606 50.5
6 01/08 Ampicillin Sodium Crystalline 2039606 65.44
7 01/08 Nutrient broth for Microbiology 2039607 500g 50
8 01/08 Cryobox Polycarbonate Yellow Grid 133mmx133mmx51mm 2039608 2ml 29.51
9 01/08 Cryobox Polycarbonate Red Grid 133mmx133mmx51mm 2039608 2ml 29.51
10 01/08 Gene Pulser/Micropulser Cuvettes 0.1cm Gap 2039609 126.35
11 01/08 Delivery charge 2039609 12.5
12 01/08 EcoR1 2039610 34
13 01/08 Xba1 2039610 33
14 01/08 Spe1 2039610 34
15 01/08 Pst1 2039610 42
16 01/08 T4 DNA Ligase 2039610 48 835.56
17 04/08 SYBR Safe DNA gel stain 10 000X concentrate in DMSO 2040668 36.1
18 04/08 N-(-B-Ketocaproyl)-L-Homoserine Lactone 2040669 29.61 901.27
19 09/08 L-Phosphatidic acid sodium from Egg Yolk 2042549 1 10.92
20 09/08 Mineral Oil Molecular Biology Reagent 2042549 500ml 18.35
21 09/08 1,2-Dioleoyl-Sn-glycero-3-phosphocholine 2042549 5ml 53.70
22 09/08 L-Amino Acids Kit 2042549 1 203
23 09/08 T7 RNA Polymerase Recombinant; Expressed 2042549 10mg/ml 29.9
24 09/08 Adenosine 5-Triphosphate Standard *Disodium 2042549 5vl 63.82
25 09/08 Phospho(enol)pyruvis acid monopotassium 2042549 1g 141.07
26 09/08 Pyruvate Kinase Tpe VIII from Bacillus 2042549 1 135.51
27 09/08 Isopropyl B-D-Thiogalactopyranoside *Dioxane 2042549 5g 121.50
28 09/08 Magnesium Acetate Solution, for Molecular Biology 2042549 500ml 120
29 09/08 L-Glutamic Acid Monopotassium 2042549 500g 66.88
30 09/08 YT Microbial Medium 2042549 250g 22.70 1888.62
31 09/08 Geneart 125.09 2013.71
32 14/08 E.coli S30 Extract System for Circular DNA (Promega) L1020 30 reactions 139
33 14/08 E.coli T7 S30 Extract System for Circular DNA (Promega) L1130 30 reactions 139
34 14/08 Nuclease free water (Promega) - 2 x 25µL 21
35 14/08 Delivery charges - - 15 2202.62
36 14/08 Red Filter - 1 140
37 14/08 Red Filter - 1 198
38 14/08 Delivery charges - - 10 2550.62
39 17/08 Oligos(MWG) - - 15.64
40 17/08 Sealing mats(Fischer) - - 16.00
41 21/08 ATP A7699 1g 38.60
42 21/08 CTP 30320 100mg 34.70
43 21/08 GTP G8877 25mg 17.70
44 21/08 UTP 94370 250mg 23.70
45 21/08 E.coli tRNA R4251 500UN 52.60
46 21/08 cAMP 01895 100mg 13.40
47 21/08 Folinic acid F8259 25mg 21.00 2783.96
48 28/8 Recombinant GFP (Clontech) QV071299 1 UN 221.25 (25% discount)
49 28/8 Recombinant DsRED Express (Clontech) QV071299 1 UN 224.25 (25% discount)
50 28/8 Delivery for Clontech - - 30
51 28/8 Qiagen Maxi Kit Cat no.12162 1 107.10
52 28/8 Delivery Charges - 12.00
53 28/8 L-A-Phosphatidyl Choline B Palmitoyl-*GA - 1 UN 140.50
54 28/8 Sorbitan Monooleate - 1 UN 10.08
55 28/8 Dodecane. Anhydrous, 99+ - 1 UN 28.60 3557.74
56 29/8 E.coli S30 Extract System for Circular DNA (Promega) L1020 1 UN 139.00
57 29/8 Delivery charges - - 15 3711.74
58 03/09 E.coli S30 Extract System for Circular DNA (Promega) L1020 3 UN 417.00
59 03/09 Delivery Charges - - 15
60 03/09 Dodecane - 500ml 84.00
61 03/09 Delivery - - 13.00 4240.74
62 04/10 Sequencing of DNA constructs - - 170.00
63 04/10 E.coli S30 Extract System for Circular DNA (Promega) L1020 2 UN 278.00

Acknowledgements

  1. Geneart is giving us 10000 euros worth of products.
  2. Promega gave us a 50% discount for the all of the E.coli S30 extract system and E.coli T7 S30 extract system for circular DNA we have purchsed.
  3. Clontech is giving us 25% discount for all our purchases made.
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