IGEM:IMPERIAL/2007/Tutorials/Guide for Engineers

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
== Introduction ==
+
== Background ==
Engineers don't normally possess a detailed knowledge of biochemistry and cell biology. One of the challenges they face when entering the iGEM project is how to start learning about the biological concepts that are involved in synthetic biology.  
Engineers don't normally possess a detailed knowledge of biochemistry and cell biology. One of the challenges they face when entering the iGEM project is how to start learning about the biological concepts that are involved in synthetic biology.  
Line 6: Line 6:
-
== Sections ==
+
== Biological Concepts ==
-
* Central Dogma of Molecular Biology
+
* [[IGEM:IMPERIAL/2007/Tutorials/Guide for Engineers/Central Dogma of Molecular Biology|Central Dogma of Molecular Biology]]
-
** The Basics: Cell Organization and DNA Structure
+
** [[Media:icgems_centraldogma1.ppt|PPT Part I]]
-
** DNA Replication
+
*** Topics discussed: The basics, DNA replication & transcription
-
** Transcription
+
** [[Media:icgems_centraldogma2.ppt|PPT Part II]]
-
*** ''Lac'' Operon
+
*** Topics discussed: Translation, protein trafficking and cell-cell communications & Criticisms of the central dogma
-
** Translation
+
-
** Protein Trafficking
+
-
** Cell-to-cell Communication
+
-
*** Quorum Sensing
+
-
** Criticisms of Central Dogma and Alternative Thinking
+
-
* Standard Laboratory Techniques
+
* [[IGEM:IMPERIAL/2007/Tutorials/Guide for Engineers/Standard Lab Techniques|Standard Laboratory Techniques]]
-
** The Molecular Biologist Toolbox Kit
+
** [[Media:icgems_labtech.ppt|PPT]]
-
*** Restriction Enzymes
+
*** Topics discussed: Restriction enzymes, gel electrophoresis, blotting techniques-Southern, Northern and Western, DNA sequencing, polymerase chain reaction (PCR), recombinant DNA & gene cloning
-
*** DNA polymerase
+
-
*** DNA ligase
+
-
*** Vectors and Plasmids
+
-
** Recombinant DNA (Gene Cloning)
+
-
** PCR (Polymerase Chain Reaction)
+
-
** Gel Electrophoresis
+
-
** Blotting Techniques
+
-
*** Western Blot
+
-
*** Northern Blot
+
-
*** Southern Blot
+
-
** DNA Sequencing
+
-
** ??
+
* [[IGEM:IMPERIAL/2007/Tutorials/Guide for Engineers/Glossary of Terms| Glossary of Useful Terms]]
* [[IGEM:IMPERIAL/2007/Tutorials/Guide for Engineers/Glossary of Terms| Glossary of Useful Terms]]
 +
 +
== Questions for Biologists ==
 +
 +
Unsure about concepts or having an insatiable desire to know more about biology? Questions and comments can be posted and discussed here.

Revision as of 20:22, 12 July 2007

Background

Engineers don't normally possess a detailed knowledge of biochemistry and cell biology. One of the challenges they face when entering the iGEM project is how to start learning about the biological concepts that are involved in synthetic biology.

The aim of this guide is to give an overview of the applicable biological concepts that will be useful for engineers engaging in synthetic biology. It is compiled by the biologists of the iGEM team for the engineers in a hope that the reciprocal exchange of knowledge and expertise would greatly benefit the team in future brainstorming sessions and experimental design and planning.


Biological Concepts


  • Standard Laboratory Techniques
    • PPT
      • Topics discussed: Restriction enzymes, gel electrophoresis, blotting techniques-Southern, Northern and Western, DNA sequencing, polymerase chain reaction (PCR), recombinant DNA & gene cloning


Questions for Biologists

Unsure about concepts or having an insatiable desire to know more about biology? Questions and comments can be posted and discussed here.

Personal tools