IGEM:IMPERIAL/2007/Tutorials/Guide for Engineers

Introduction

Engineers don't normally possess a detailed knowledge of biochemistry and cell biology. One of the challenges they face when entering the iGEM project is how to start learning about the biological concepts that are involved in synthetic biology.

The aim of this guide is to give an overview of the applicable biological concepts that will be useful for engineers engaging in synthetic biology. It is compiled by the biologists of the iGEM team for the engineers in a hope that the reciprocal exchange of knowledge and expertise would greatly benefit the team in future brainstorming sessions and experimental design and planning.

Sections

• Central Dogma of Molecular Biology
  • The Basics: Cell Organization and DNA Structure
  • DNA Replication
  • Transcription
    • Lac Operon
  • Translation
  • Protein Trafficking
  • Cell-to-cell Communication
    • Quorum Sensing
  • Criticisms of Central Dogma and Alternative Thinking

• Standard Laboratory Techniques
  • The Molecular Biologist Toolbox Kit
    • Restriction Enzymes
    • DNA polymerase
    • DNA ligase
    • Vectors and Plasmids
  • Recombinant DNA (Gene Cloning)
  • PCR (Polymerase Chain Reaction)
  • Gel Electrophoresis
  • Blotting Techniques
    • Western Blot
    • Northern Blot
    • Southern Blot
  • DNA Sequencing
  • ??

• Glossary of Useful Terms