IGEM:IMPERIAL/2007/Week5: Difference between revisions

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<br>'''By end of week:''' Build complete DNA constructs<br>
<br>'''By end of week:''' Build complete DNA constructs<br>


*'''Merge protocols''': James, Peixuan, Lucas
*'''Merge protocols''': James, Peixuan, Lucas
Line 36: Line 37:


<br>'''By end of week:''' Efficient planning pf protocols and lab sessions<br>
<br>'''By end of week:''' Efficient planning pf protocols and lab sessions<br>


*'''Learn vessicles:''' Dirk, Cheuk
*'''Learn vessicles:''' Dirk, Cheuk
Line 47: Line 49:


<br>'''By end of this week:''' Finalise protocols, Wait for material delivery <br>
<br>'''By end of this week:''' Finalise protocols, Wait for material delivery <br>


*'''Wiki:''' Everyone
*'''Wiki:''' Everyone
Line 58: Line 61:


<br>'''By end of week:''' Get supervisors back into the 'loop'. Get organised <br>
<br>'''By end of week:''' Get supervisors back into the 'loop'. Get organised <br>


*'''Modelling:''' Jerry, Alex
*'''Modelling:''' Jerry, Alex
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|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Alex  
| style="background:#aabadd" | Alex  
|| ||  ||  ||  ||  ||  ||
|| Define Std Unit ||  ||  ||  ||  ||  ||
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Anthony  
| style="background:#aabadd" | Anthony  
|| ||  ||  ||  ||  ||  ||
|| Define Std Unit ||  ||  ||  ||  ||  ||
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Ben  
| style="background:#aabadd" | Ben  
|| ||  ||  ||  ||  ||  ||
|| Prep/Cloning ||  ||  ||  ||  ||  ||
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Cheuk  
| style="background:#aabadd" | Cheuk  
|| ||  ||  ||  ||  ||  ||
|| Definition/Logo||  ||  ||  ||  ||  ||
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Dirk  
| style="background:#aabadd" | Dirk  
|| ||  ||  ||  ||  ||  ||
|| Learn Vesicles||  ||  ||  ||  ||  ||
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | James  
| style="background:#aabadd" | James  
|| ||  ||  ||  ||  ||  ||
|| Define Std Unit ||  ||  ||  ||  ||  ||
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Jerry  
| style="background:#aabadd" | Jerry  
|| ||  ||  ||  ||  ||  ||
|| Modelling ||  ||  ||  ||  ||  ||
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Lucas  
| style="background:#aabadd" | Lucas  
|| ||  ||  ||  ||  ||  ||
|| Prep/Cloning ||  ||  ||  ||  ||  ||
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Maira  
| style="background:#aabadd" | Maira  
|| ||  ||  ||  ||  ||  ||
|| Prep/Cloning ||  ||  ||  ||  ||  ||
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Peixuan  
| style="background:#aabadd" | Peixuan  
|| ||  ||  ||  ||  ||  ||
|| Define Std Unit ||  ||  ||  ||  ||  ||
|}
|}


Revision as of 16:28, 6 August 2007

<<Previous Week| Mon| Tue| Wed| Thu| Fri| Sat| Sun| Next Week>>
 

For the Weekend

This Week's Aims

  • Wet lab: Cheuk, Maira, Ben
    • Cloning:
      1. sort out DNA constructs to build
      2. transform cells
      3. amplify biobricks
      4. midi/maxi preps
      5. digestion and ligation
      6. build complete DNA constructs
    • In vitro:
      1. wait for materials/look for BL21
      2. prepare cell extracts


By end of week: Build complete DNA constructs


  • Merge protocols: James, Peixuan, Lucas
    • Define standard units
    • Learn chassis
    • Learn cell-by-date
    • Learn biofilm
      1. merege protocols (prioritise)
      2. provisional lab schedule
      3. learn biofilm production


By end of week: Efficient planning pf protocols and lab sessions


  • Learn vessicles: Dirk, Cheuk
    • Read up papers
      1. materials
      2. equipment
      3. compare results of different techniques
    • Contact people
      1. focus on techniques
      2. ask for opinion


By end of this week: Finalise protocols, Wait for material delivery


  • Wiki: Everyone
    • Have a schedule
      1. weekly
      2. daily
    • Updated project page
      1. progress throughout each day
      2. progress throughout each week
    • Have an iGEM 'digest' for the professors and supervisors (email)


By end of week: Get supervisors back into the 'loop'. Get organised


  • Modelling: Jerry, Alex
    • Learn biofilm
      1. diffusion process within biofilms
      2. [AHL] and temperature dependence
    • Learn cell-by-date
      1. temperature dependence
      2. degradation time
      3. steady state (rate of formation/decay of GFP)


By end of week: Model basic constructs and parameters to be measured

Weekly Outlook

Name Monday Tuesday Wednesday Thursday Friday Saturday Sunday
Alex Define Std Unit
Anthony Define Std Unit
Ben Prep/Cloning
Cheuk Definition/Logo
Dirk Learn Vesicles
James Define Std Unit
Jerry Modelling
Lucas Prep/Cloning
Maira Prep/Cloning
Peixuan Define Std Unit


Group Timetable

Day Monday Tuesday Wednesday Thursday Friday Saturday Sunday
0900 Daily
Intro		 Daily
Intro		 Daily
Intro		 Daily
Intro		 Daily
Intro
0930 Journal Club Journal Club Journal Club Journal Club Journal Club
1000 = = = = =
1030
1100
1130
1200 Lunch Lunch Lunch Lunch Lunch
1230 = = = = =
1300
1330
1400
1430
1500
1530
1600
1630
1700 Debriefing Debriefing Debriefing Debriefing Debriefing
1730 Wikification Wikification Wikification Wikification Wikification
1800
Evening

Expenses

Name Expenditure Description
Total: £0.00


 
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