IGEM:IMPERIAL/2007/Wet Lab/Protocols

Protocols

Below is a databank of all the protocols that have been used in our projects.

Fabrication Protocols

Testing Protocols

General Protocols Cloning of DNA- MiniPrep Cloning of DNA- MidiPrep Cloning of DNA- MaxiPrep Purification of GFP Fluorometer- Optimum Exposure Time Volume dependance of Fluorescence GFP vs Fluorescence Calibration Curve Degradation Time of GFP Purification of LuxR Chassis Protocols E. coli Extracts Preparation of S30 E. coli extract Preparation of S12 E. coli extract Preparation of reaction mixture Vesicles Formation 1 2

Cell by Date Phase 1 Initial Testing for DNA constructs of CBD In Vivo Initial Testing for DNA constructs of CBD In Vitro Problems with Evaporation over a 24 hour period Phase 2 Operating Temperature Range (Initial Tests) 2 Phase 3 1 2 Infector Detector Phase 1 Initial Testing for DNA constructs of ID In Vivo Initial Testing for DNA constructs of ID In Vitro Phase 2 Preliminary AHL Testing AHL Sensitivity: Real Life Applications Phase 3 1 2

Click here for the protocols template.