IGEM:IMPERIAL/2007/Wet Lab/Protocols: Difference between revisions

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__NOTOC__
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{| style="width:800px; height:80px;" cellpadding="0" border="0"
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| width="220px" | <html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html>
| width="390px" |  <br><center><html><h3>THE IMPERIAL iGEM 2007 Team </h3></html></center>
| width="110px"| <html><a href="http://www.imperial.ac.uk/centenary"><p align="right"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/7813700.GIF" height="60"></p></a></html>
|}
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{| style="width:800px;" cellpadding="2" cellspacing="0" align="center"
|- style="background:#000000; color:white;" align="center"
| [[IGEM:IMPERIAL/2007|<font face="Arial" size=1 style="color:#ffffff"> '''Home'''</font>]]
| [[IGEM:IMPERIAL/2007/Projects/Cell By Date|<font face="Arial" size=1 style="color:#ffffff"> '''Cell By Date'''</font>]]
| [[IGEM:IMPERIAL/2007/Biofilm Detector|<font face="Arial" size=1 style="color:#ffffff"> '''Infector Detector'''</font>]]
| [[IGEM:IMPERIAL/2007/Wet Lab|<font face="Arial" size=1 style="color:#ffffff"> '''Wet Lab'''</font>]]
| [[IGEM:IMPERIAL/2007/Dry Lab|<font face="Arial" size=1 style="color:#ffffff"> '''Dry Lab'''</font>]]
| [[IGEM:IMPERIAL/2007/Resources|<font face="Arial" size=1 style="color:#ffffff">'''Resources'''</font>]]
| [[IGEM:IMPERIAL/2007/Meet The Team|<font face="Arial" size=1 style="color:#ffffff"> '''Meet the
Team'''</font>]]
| [[IGEM:IMPERIAL/2007/Entertainment|<font face="Arial" size=1 style="color:#ffffff"> '''Entertainment'''</font>]]
| [[IGEM:IMPERIAL/2007/Acknowledgments|<font face="Arial" size=1 style="color:#ffffff"> '''Acknowledgments'''</font>]]
|}
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| {{Click || image=Service_course.png | link=IGEM:IMPERIAL/2007/Wet Lab/Lab Notebook| width=40px | height=40px }}
| [[IGEM:IMPERIAL/2007/Wet Lab/Lab Notebook|<font face="Arial" size=2 style="color:#000099">'''Lab Notebook'''</font>]]<br /> <font size=1>Daily Record of Wet Lab</font>
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| <br>
|- valign="center"
| {{Click || image=Service_protocols.png | link=IGEM:IMPERIAL/2007/Wet Lab/Protocols| width=40px | height=40px }}
| [[IGEM:IMPERIAL/2007/Wet Lab/Protocols|<font face="Arial" size=2 style="color:#000099">'''Protocols'''</font>]]<br /> <font size=1>Fabrication & Testing Protocols</font>
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| <br>
|- valign="center"
| {{Click || image=Service_notebook.png | link=IGEM:IMPERIAL/2007/Wet Lab/DNA Constructs| width=40px | height=40px }}
| [[IGEM:IMPERIAL/2007/Wet Lab/DNA Constructs|<font face="Arial" size=2 style="color:#000099">'''DNA Constructs'''</font>]]<br /> <font size=1>Overview of DNA Constructs</font>
|-
| <br>
|- valign="center"
| {{Click || image=Scientificwriters.png | link=IGEM:IMPERIAL/2007/Wet Lab/Shopping List| width=40px | height=40px }}
| [[IGEM:IMPERIAL/2007/Wet Lab/Shopping List|<font face="Arial" size=2 style="color:#000099">'''Shopping List'''</font>]]<br /> <font size=1>Expenditures on Materials</font>
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== Protocols ==
== Protocols ==
Below is a databank of all the protocols that have been used in our projects.  
Below is a databank of all the protocols that have been used in our projects.  
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|- style="background:#aabadd; color:Black" align="left"  
 
|| <font size=4>'''Fabrication Protocols'''</font>|| <font size=3>'''Testing Protocols'''</font>
 
{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left"
|- style="background:#aabadd; color:Black" align="center"  
|width="35%"|<font size=2>'''General Experiments'''</font>||width="65%"|<font size=2>'''Testing Experiments'''</font>
|- style="background:#d5dcef; color:Black" valign="top"
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<font size=3>'''General Protocols'''</font> <br>
<font size=2><font color=green>'''Protein Purification'''</font> <br>
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/DNA1.1|Cloning of DNA- MiniPrep]]
* ''Purification of LuxR'' : [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.5|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.5|Results]]
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/DNA1.2|Cloning of DNA- MidiPrep]]
* ''Purification of GFPmut3b'': 
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/DNA1.3|Cloning of DNA- MaxiPrep]]
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.6|Results]]
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.1|Fluorometer- Optimum Exposure Time]]
<br>
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.2|Volume dependance of Fluorescence]]
<font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.2|GFP vs Fluorescence Calibration Curve]]
* ''Optimisation of Fluorometer'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.1|Results]]<br>
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.2|Degradation Time of GFP]]
<font size=2><font color=green>'''Calibration Curve'''</font> <br>
* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.3|Results]]
* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.4|Results]]
<br>
<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
* ''Preparation S30 Cell Extract '':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.1|Protocol]]
* ''Preparation S12 Cell Extract '':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.2|Protocol]]
* ''Preparation of Reaction Mixture'':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.3|Protocol]]
* ''Vesicles Formation'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/VF1.1|Protocol]]


<br>
|
<font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
*''pT7 in vitro:'' Protocols and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.9|Results]]


<font size=2>'''Cell by Date'''</font>
<br>
<font color=green>'''Implementation'''</font>
<br>
<br>
<font size=3>'''Chassis Protocols'''</font> <br>
* ''In Vivo Testing:'' [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD1.1|Results]]
''' S30 ''E. coli'' Extract'''<br>
* ''Initial In vitro Testing'':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.2|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD1.2|Results]]
* 1
<font color=green>'''Testing/Validation<br>'''</font>
* 2
*''Testing DNA Concentration in vitro'': Protocol and [[IGEM:Imperial/2007/Wet_Lab/results/CBD4 |Results]]
* ''Operating Temperature Range'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and  [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and  [[IGEM:Imperial/2007/Wet_Lab/results/CBD2.2|Results]]
*''Temperature Steps:'' Protocols and Results
 
<font size=2>'''Infecter Detector'''</font> <br>
<font color=green>'''Implementation<br>'''</font>
*''In vivo Testing'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID1.1|Results]]
* ''Initial In Vitro Testing'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID1.2|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID1.2|Results]]
<font color=green>'''Testing/Validation<br>'''</font>
*''Testing of DNA concentration'' : Protocol and [[IGEM:Imperial/2007/Wet_Lab/results/Res2.1|Results]]
* ''Testing of Purified LuxR'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.7|Results]]
* ''Characterization in vitro - AHL titration '': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID3.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID3.1|Results]]
|}
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'''Vesicles Formation'''<br>
* 1
* 2


|
<font size=3>'''Cell by Date'''</font> <br>
'''Phase 1'''<br>
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.1|Initial Testing for DNA constructs of CBD In Vivo]]
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.2|Initial Testing for DNA constructs of CBD In Vitro]]


OLD RESULTS + PROTOCOLS


<font size=3>'''Fluorometer Testing'''</font> <br>


'''Phase 2'''<br>
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.5|Measuring and Preventing Evaporation]]
* 1
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.2|Testing How Fluorescence Changes With Volume]]
* 2
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.3|Calibration Curve for DsRed express]]
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.4|Degradation Curve]]
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.8|DNA concentration experiments]]


'''Phase 3'''<br>
* 1
* 2


<br>
<br>
<font size=3>'''Infector Detector'''</font> <br>
<font size=3>'''Infector Detector'''</font> <br>
'''Phase 1'''<br>
'''Phase 1'''<br>
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID1.1|Initial Testing for DNA constructs of ID In Vivo]]
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID1.2|Initial Testing for DNA constructs of ID In Vitro]]


'''Phase 2'''<br>
'''Phase 2'''<br>
* 1
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.1|Preliminary AHL Testing]]
* 2
 
'''Phase 3'''<br>
* 1
* 2
 
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Click [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Template|here]] for the protocols template.
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.2|AHL Sensitivity: Real Life Applications]]

Latest revision as of 07:38, 25 October 2007

<html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html>
<html><h3>THE IMPERIAL iGEM 2007 Team </h3></html>
 <html><a href="http://www.imperial.ac.uk/centenary"><p align="right"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/7813700.GIF" height="60"></p></a></html>
Home Cell By Date Infector Detector Wet Lab Dry Lab Resources Meet the Team Entertainment Acknowledgments


Lab Notebook
Daily Record of Wet Lab

Protocols
Fabrication & Testing Protocols

DNA Constructs
Overview of DNA Constructs

Shopping List
Expenditures on Materials


Protocols

Below is a databank of all the protocols that have been used in our projects.

General Experiments Testing Experiments

Protein Purification


Fluorometer Testing

Calibration Curve


Chassis Protocols


pT7 Characterisation

  • pT7 in vitro: Protocols and Results

Cell by Date
Implementation

Testing/Validation

Infecter Detector
Implementation

Testing/Validation







OLD RESULTS + PROTOCOLS

Fluorometer Testing



Infector Detector
Phase 1

Phase 2

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