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| width="220px" | <html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html> | |||
| width="390px" | <br><center><html><h3>THE IMPERIAL iGEM 2007 Team </h3></html></center> | |||
| width="110px"| <html><a href="http://www.imperial.ac.uk/centenary"><p align="right"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/7813700.GIF" height="60"></p></a></html> | |||
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| [[IGEM:IMPERIAL/2007|<font face="Arial" size=1 style="color:#ffffff"> '''Home'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Projects/Cell By Date|<font face="Arial" size=1 style="color:#ffffff"> '''Cell By Date'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Biofilm Detector|<font face="Arial" size=1 style="color:#ffffff"> '''Infector Detector'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Wet Lab|<font face="Arial" size=1 style="color:#ffffff"> '''Wet Lab'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Dry Lab|<font face="Arial" size=1 style="color:#ffffff"> '''Dry Lab'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Resources|<font face="Arial" size=1 style="color:#ffffff">'''Resources'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Meet The Team|<font face="Arial" size=1 style="color:#ffffff"> '''Meet the | |||
Team'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Entertainment|<font face="Arial" size=1 style="color:#ffffff"> '''Entertainment'''</font>]] | |||
| [[IGEM:IMPERIAL/2007/Acknowledgments|<font face="Arial" size=1 style="color:#ffffff"> '''Acknowledgments'''</font>]] | |||
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| {{Click || image=Service_course.png | link=IGEM:IMPERIAL/2007/Wet Lab/Lab Notebook| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/Lab Notebook|<font face="Arial" size=2 style="color:#000099">'''Lab Notebook'''</font>]]<br /> <font size=1>Daily Record of Wet Lab</font> | |||
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| {{Click || image=Service_protocols.png | link=IGEM:IMPERIAL/2007/Wet Lab/Protocols| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/Protocols|<font face="Arial" size=2 style="color:#000099">'''Protocols'''</font>]]<br /> <font size=1>Fabrication & Testing Protocols</font> | |||
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| {{Click || image=Service_notebook.png | link=IGEM:IMPERIAL/2007/Wet Lab/DNA Constructs| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/DNA Constructs|<font face="Arial" size=2 style="color:#000099">'''DNA Constructs'''</font>]]<br /> <font size=1>Overview of DNA Constructs</font> | |||
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| {{Click || image=Scientificwriters.png | link=IGEM:IMPERIAL/2007/Wet Lab/Shopping List| width=40px | height=40px }} | |||
| [[IGEM:IMPERIAL/2007/Wet Lab/Shopping List|<font face="Arial" size=2 style="color:#000099">'''Shopping List'''</font>]]<br /> <font size=1>Expenditures on Materials</font> | |||
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== Protocols == | == Protocols == | ||
Below is a databank of all the protocols that have been used in our projects. | Below is a databank of all the protocols that have been used in our projects. | ||
<br><br> | <br><br> | ||
{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left" | |||
{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left" | {| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left" | ||
|- style="background:#aabadd; color:Black" align="center" | |- style="background:#aabadd; color:Black" align="center" | ||
|| <font size= | |width="35%"|<font size=2>'''General Experiments'''</font>||width="65%"|<font size=2>'''Testing Experiments'''</font> | ||
|- style="background:#d5dcef; color:Black" valign="top" | |- style="background:#d5dcef; color:Black" valign="top" | ||
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<font size=3>''' | <font size=2><font color=green>'''Protein Purification'''</font> <br> | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ | * ''Purification of LuxR'' : [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.5|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.5|Results]] | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ | * ''Purification of GFPmut3b'': | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ | * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.6|Results]] | ||
<br> | |||
<font size=2><font color=green>'''Fluorometer Testing'''</font> <br> | |||
* ''Optimisation of Fluorometer'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.1|Results]]<br> | |||
<font size=2><font color=green>'''Calibration Curve'''</font> <br> | |||
* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.3|Results]] | |||
* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.4|Results]] | |||
<br> | |||
<font size=2><font color=green>'''Chassis Protocols'''</font> <br> | |||
* ''Preparation S30 Cell Extract '':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.1|Protocol]] | |||
* ''Preparation S12 Cell Extract '':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.2|Protocol]] | |||
* ''Preparation of Reaction Mixture'':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.3|Protocol]] | |||
* ''Vesicles Formation'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/VF1.1|Protocol]] | |||
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<font size=2><font color=green>'''pT7 Characterisation'''</font> <br> | |||
*''pT7 in vitro:'' Protocols and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.9|Results]] | |||
<font size=2>'''Cell by Date'''</font> | |||
<br> | <br> | ||
<font size= | <font color=green>'''Implementation'''</font> | ||
<br> | |||
* ''In Vivo Testing:'' [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD1.1|Results]] | |||
* ''Initial In vitro Testing'':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.2|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD1.2|Results]] | |||
<font color=green>'''Testing/Validation<br>'''</font> | |||
*''Testing DNA Concentration in vitro'': Protocol and [[IGEM:Imperial/2007/Wet_Lab/results/CBD4 |Results]] | |||
* ''Operating Temperature Range'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD2.2|Results]] | |||
*''Temperature Steps:'' Protocols and Results | |||
<font size=2>'''Infecter Detector'''</font> <br> | |||
<font color=green>'''Implementation<br>'''</font> | |||
*''In vivo Testing'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID1.1|Results]] | |||
* ''Initial In Vitro Testing'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID1.2|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID1.2|Results]] | |||
<font color=green>'''Testing/Validation<br>'''</font> | |||
*''Testing of DNA concentration'' : Protocol and [[IGEM:Imperial/2007/Wet_Lab/results/Res2.1|Results]] | |||
* ''Testing of Purified LuxR'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.7|Results]] | |||
* ''Characterization in vitro - AHL titration '': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID3.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID3.1|Results]] | |||
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OLD RESULTS + PROTOCOLS | |||
''' | <font size=3>'''Fluorometer Testing'''</font> <br> | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ | |||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ | * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.5|Measuring and Preventing Evaporation]] | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.2|Testing How Fluorescence Changes With Volume]] | |||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.3|Calibration Curve for DsRed express]] | |||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.4|Degradation Curve]] | |||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.8|DNA concentration experiments]] | |||
<br> | <br> | ||
<font size=3>'''Infector Detector'''</font> <br> | <font size=3>'''Infector Detector'''</font> <br> | ||
'''Phase 1'''<br> | '''Phase 1'''<br> | ||
'''Phase 2'''<br> | '''Phase 2'''<br> | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.1|Preliminary AHL Testing]] | * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.1|Preliminary AHL Testing]] | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.2|AHL Sensitivity: Real Life Applications]] | * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.2|AHL Sensitivity: Real Life Applications]] | ||
Latest revision as of 07:38, 25 October 2007
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Home | Cell By Date | Infector Detector | Wet Lab | Dry Lab | Resources | Meet the Team | Entertainment | Acknowledgments |
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ProtocolsBelow is a databank of all the protocols that have been used in our projects.
Fluorometer Testing
Phase 2 | |||||||||||||||