IGEM:IMPERIAL/2007/Wet Lab/Protocols: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
 
(6 intermediate revisions by 2 users not shown)
Line 55: Line 55:


{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left"
{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left"
= Wet Lab: Protocols =
Below is a databank of all the protocols that have been used in our projects.
<br><br>




{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left"
{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left"
|- style="background:#aabadd; color:Black" align="center"  
|- style="background:#aabadd; color:Black" align="center"  
||<font size=2>'''General Experiments'''</font>||<font size=2>'''Testing Experiments'''</font>
|width="35%"|<font size=2>'''General Experiments'''</font>||width="65%"|<font size=2>'''Testing Experiments'''</font>
|- style="background:#d5dcef; color:Black" valign="top"
|- style="background:#d5dcef; color:Black" valign="top"
|
|
Line 81: Line 77:
* ''Preparation S12 Cell Extract '':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.2|Protocol]]
* ''Preparation S12 Cell Extract '':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.2|Protocol]]
* ''Preparation of Reaction Mixture'':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.3|Protocol]]
* ''Preparation of Reaction Mixture'':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.3|Protocol]]
* ''Vesicles Formation'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/VF1.1|Vesicles Formation|Protocol]]
* ''Vesicles Formation'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/VF1.1|Protocol]]


<br>
<br>
Line 106: Line 102:
*''Testing of DNA concentration'' : Protocol and [[IGEM:Imperial/2007/Wet_Lab/results/Res2.1|Results]]
*''Testing of DNA concentration'' : Protocol and [[IGEM:Imperial/2007/Wet_Lab/results/Res2.1|Results]]
* ''Testing of Purified LuxR'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.7|Results]]
* ''Testing of Purified LuxR'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.7|Results]]
* ''Characterization in vitro - AHL titration '': [[* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID3.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID3.1|Results]]
* ''Characterization in vitro - AHL titration '': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID3.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID3.1|Results]]
|}
|}
<br clear="all">
<br clear="all">

Latest revision as of 07:38, 25 October 2007

<html><a href="http://www.imperial.ac.uk"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/281904.GIF" height="55"></a></html>
<html><h3>THE IMPERIAL iGEM 2007 Team </h3></html>
<html><a href="http://www.imperial.ac.uk/centenary"><p align="right"><img src="http://www3.imperial.ac.uk/portal/pls/portallive/docs/1/7813700.GIF" height="60"></p></a></html>
Home Cell By Date Infector Detector Wet Lab Dry Lab Resources Meet the Team Entertainment Acknowledgments


Lab Notebook
Daily Record of Wet Lab

Protocols
Fabrication & Testing Protocols

DNA Constructs
Overview of DNA Constructs

Shopping List
Expenditures on Materials


Protocols

Below is a databank of all the protocols that have been used in our projects.

General Experiments Testing Experiments

Protein Purification


Fluorometer Testing

Calibration Curve


Chassis Protocols


pT7 Characterisation

  • pT7 in vitro: Protocols and Results

Cell by Date
Implementation

Testing/Validation

Infecter Detector
Implementation

Testing/Validation







OLD RESULTS + PROTOCOLS

Fluorometer Testing



Infector Detector
Phase 1

Phase 2