IGEM:IMPERIAL/2007/Wet Lab/Protocols

Protocols

Below is a databank of all the protocols that have been used in our projects.

Fabrication Protocols

Testing Protocols

General Protocols Cloning of DNA- MiniPrep Cloning of DNA- MidiPrep Cloning of DNA- MaxiPrep Purification of GFP Fluorometer- Optimum Exposure Time Volume dependance of Fluorescence GFP vs Fluorescence Calibration Curve Degradation Time of GFP Purification of LuxR Problems with Evaporation over a 24 hour period Chassis Protocols E. coli Extracts Preparation of S30 E. coli extract Preparation of S12 E. coli extract Preparation of reaction mixture Vesicles Formation 1 2

Cell by Date Phase 1 Initial Testing for DNA constructs of CBD In Vivo Initial Testing for DNA constructs of CBD In Vitro Phase 2 Operating Temperature Range 2 Phase 3 1 2 Infector Detector Phase 1 Initial Testing for DNA constructs of ID In Vivo Initial Testing for DNA constructs of ID In Vitro Phase 2 Preliminary AHL Testing AHL Sensitivity: Real Life Applications Phase 3 1 2

Click here for the protocols template.