IGEM:IMPERIAL/2007/Wet Lab/Protocols

Protocols

Below is a databank of all the protocols that have been used in our projects.

General Protocols

Testing Protocols

Fabrication Protocols Cloning of DNA- MiniPrep Cloning of DNA- MidiPrep Cloning of DNA- MaxiPrep Protein Purification Purification of LuxR Testing of Purified LuxR Purification of mut3bGFP Fluorometer Testing Optimsation of Fluorometer Measuring and Preventing Evaporation Testing How Fluorescence Changes With Volume Calibration Curve for DsRed express Degradation Curve Chassis Protocols E. coli Extracts Preparation of S30 E. coli extract Preparation of S12 E. coli extract Preparation of reaction mixture Vesicles Formation Vesicles Formation 2

Cell by Date Phase 1 Initial Testing for DNA constructs of CBD In Vivo Initial Testing for DNA constructs of CBD In Vitro Phase 2 Operating Temperature Range (Initial Tests) Operating Temperature Range (Refined Tests) Phase 3 1 2 Infector Detector Phase 1 Initial Testing for DNA constructs of ID In Vivo Initial Testing for DNA constructs of ID In Vitro Phase 2 Preliminary AHL Testing AHL Sensitivity: Real Life Applications Phase 3 1 2

Click here for the protocols template.