IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.2

Operating Temperature Range (Refined Testing)

Aims:

• To measure the fluorescence over a 6 hour period, for a range of temperatures.
• To continue from the temperatures measured before, 37oC and 20oC; temperatures 8oC, 15oC, 25oC and 30oC are measured this time round.
• 8oC is the hypothesized minimum temperature where the system stops working. Judging from the behaviour of the system at this temperature, we will refine its working parameters.

Equipment

• 4 Water Baths
• Cold Room
• Fluorometer connected to a PC
• Thermometers
• 4 96 black Well Plates
• Eppendorf Tubes
• Gilson Pipettes p1000, p200, p20

Reagents

• S30 Commercial Cell Extract for 16 reactions
• DNA pTet-GFP from maxiprep
• Nuclease Free Water