IGEM:IMPERIAL/2008/New/BioBricks: Difference between revisions

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|- style=background:red
|- style=background:red
| K143008 || 5' PyrD Integration Sequence || Awaiting GeneArt delivery
| K143008 || 5' PyrD Integration Sequence || Awaiting GeneArt delivery
|- style=background:royalblue
|- style=background:mediumslateblue
| K143009 || 3' PyrD Integration Sequence || Transformed 1-10
| K143009 || 3' PyrD Integration Sequence || PCR verified
|- style=background:yellow
|- style=background:yellow
| K143031 || Aad9 Spectinomycin Resistance Gene || Ligation Failed
| K143031 || Aad9 Spectinomycin Resistance Gene || Ligation Failed
Line 55: Line 55:
| K143032 || EpsE Molecular Clutch Gene of ''B. subtilis'' || Sequenced
| K143032 || EpsE Molecular Clutch Gene of ''B. subtilis'' || Sequenced
|- style=background:mediumslateblue
|- style=background:mediumslateblue
| K143033 || LacI (Lva<sup>-</sup>, N terminal deletion) || PCR verified
| K143033 || LacI (Lva<sup>-</sup>, N terminal deletion) || Digest verfied
|- style=background:#cc4400
|- style=background:#cc4400
| K143034 || LipA-EAK16 Fusion || Midiprepped
| K143034 || LipA-EAK16 Fusion || Midiprepped
|- style=background:royalblue
|- style=background:royalblue
| K143035 || LipA-Human Elastin Fusion || Transformed 25/9
| K143035 || LipA-Human Elastin Fusion || Transformed 25/9
|- style=background:royalblue
|- style=background:mediumslateblue
| K143036 || Xylose Operon Regulatory Protein || Transformed 29/9
| K143036 || Xylose Operon Regulatory Protein || PCR verified
|- style=background:royalblue
|- style=background:mediumslateblue
| K143037 || Ytva Blue Light Receptor of ''B. subtilis'' || Transformed 1-10
| K143037 || Ytva Blue Light Receptor of ''B. subtilis'' || PCR verified
|- style=background:silver
|- style=background:silver
| K143038 || SacB-EAK16 Fusion || Sequenced
| K143038 || SacB-EAK16 Fusion || Sequenced
Line 76: Line 76:
|- style=background:red
|- style=background:red
| K143054 || Phyperspank-gsiB || Awaiting GeneArt Delivery
| K143054 || Phyperspank-gsiB || Awaiting GeneArt Delivery
|- style=background:royalblue
|- style=background:mediumslateblue
| K143055 || Phyperspank-spoVG || Transformed 1-10
| K143055 || Phyperspank-spoVG || PCR verified
|- style=background:red
|- style=background:red
| K143056 || Pxyl-gsiB || Awaiting GeneArt Delivery
| K143056 || Pxyl-gsiB || Awaiting GeneArt Delivery
|- style=background:red
|- style=background:red
| K143057 || Pxyl-spoVG || Awaiting GeneArt Delivery
| K143057 || Pxyl-spoVG || Awaiting GeneArt Delivery
|- style=background:royalblue
|- style=background:rmediumslateblur
| K143058 || Pctc-gsiB || Transformed 1-10
| K143058 || Pctc-gsiB || PCR verified
|- style=background:royalblue
|- style=background:mediumslateblue
| K143059 || Pctc-spoVG || Transformed 1-10
| K143059 || Pctc-spoVG || PCR verified
|- style=background:#cc4400
|- style=background:#cc4400
| K143060 || PgsiB-gsiB || Midiprepped
| K143060 || PgsiB-gsiB || Midiprepped
|- style=background:#cc4400
|- style=background:#cc4400
| K143061 || PgsiB-spoVG || Midiprepped
| K143061 || PgsiB-spoVG || Midiprepped
|- style=background:red
|- style=background:yellow
| K143062 || LacI - Terminator || Awaiting Components (LacI)
| K143062 || LacI - Terminator || Pending
|- style=background:red
|- style=background:red
| K143063 || XylR - Terminator || Awaiting Components (XylR)
| K143063 || XylR - Terminator || Awaiting Components (XylR)
|- style=background:yellow
|- style=background:mediumslateblue
| K143064 || CAT - Terminator || Transformed 1-10
| K143064 || CAT - Terminator || PCR verified
|- style=background:red
|- style=background:red
| K143065 || Aad9 - Terminator || Awaiting Components (Aad9)
| K143065 || Aad9 - Terminator || Awaiting Components (Aad9)

Revision as of 04:38, 3 October 2008

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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_1">Why B. subtilis?</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Cloning_Strategy">Cloning Strategy</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols">Experiments & Protocols</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Major_Results">Experimental Results</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/BioBricks">BioBricks & Characterisation</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Appendices">Appendices - Code etc.</a>
       </div></ul>

</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

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BioBricks & Characterisation

Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.

Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.

<html><center><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png">| Device 1 | Device 2 | Device 3 |<br>| The System |</center></html>


Parts Submitted to the Registry

For now, here are the parts we will submit to the Registry. They can also be accessed here.

<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px" scrolling="yes"></iframe></html>


Production Status

This table shows our progress with each part:


Key
Submitted
Sequenced
Midiprepped
Fully verified
Partially Verified
Transformed
Ligation pending/Failed
Awaiting GeneArt/Cloning
Part Number Part Name Status
K143001 5' AmyE Integration Sequence Midiprepped
K143002 3' AmyE Integration Sequence Sequenced
K143005 5' EpsE Integration Sequence Awaiting Ligation
K143006 3' EpsE Integration Sequence Awaiting Ligation
K143008 5' PyrD Integration Sequence Awaiting GeneArt delivery
K143009 3' PyrD Integration Sequence PCR verified
K143031 Aad9 Spectinomycin Resistance Gene Ligation Failed
K143032 EpsE Molecular Clutch Gene of B. subtilis Sequenced
K143033 LacI (Lva-, N terminal deletion) Digest verfied
K143034 LipA-EAK16 Fusion Midiprepped
K143035 LipA-Human Elastin Fusion Transformed 25/9
K143036 Xylose Operon Regulatory Protein PCR verified
K143037 Ytva Blue Light Receptor of B. subtilis PCR verified
K143038 SacB-EAK16 Fusion Sequenced
K143050 P43-gsiB Midiprepped
K143051 P43-spoVG Midiprepped
K143052 Pveg-gsiB Midiprepped
K143053 Pveg-spoVG Midiprepped
K143054 Phyperspank-gsiB Awaiting GeneArt Delivery
K143055 Phyperspank-spoVG PCR verified
K143056 Pxyl-gsiB Awaiting GeneArt Delivery
K143057 Pxyl-spoVG Awaiting GeneArt Delivery
K143058 Pctc-gsiB PCR verified
K143059 Pctc-spoVG PCR verified
K143060 PgsiB-gsiB Midiprepped
K143061 PgsiB-spoVG Midiprepped
K143062 LacI - Terminator Pending
K143063 XylR - Terminator Awaiting Components (XylR)
K143064 CAT - Terminator PCR verified
K143065 Aad9 - Terminator Awaiting Components (Aad9)


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