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  BioBricks & Characterisation

Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.

Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.

| Device 1 | Device 2 | Device 3 |
| The System |

For now, here are the parts we hope to submit to the Registry, they can also be accessed here and at the bottom of the page is a table showing the current progress on production of each part:

Here is a table of the parts currently in production:

Part Number Part Name Status
K143001 5' AmyE Integration Sequence Ligation being verified (1st stage passed)
K143002 3' AmyE Integration Sequence Ligation being verified (1st stage passed)
K143005 5' EpsE Integration Sequence PCR cloning to be repeated
K143006 3' EpsE Integration Sequence PCR cloning to be repeated
K143008 5' PyrD Integration Sequence Awaiting GeneArt delivery
K143009 3' PyrD Integration Sequence Awaiting Ligation
K143010 Promoter ctc for B. subtilis
K143011 Promoter gsiB for B. subtilis
K143012 Promoter veg for B. subtilis
K143013 Promoter 43 for B. subtilis
K143014 Promoter Xyl for B. subtilis
K143015 Promoter hyper-spank for B. subtilis
K143020 GsiB Ribosome Binding Site (RBS) for B. subtilis
K143021 SpoVG Ribosome Binding Site (RBS) for B. subtilis
K143031 Aad9 Spectinomycin Resistance Gene Ligation Failed
K143032 EpsE Molecular Clutch Gene of B. subtilis Ligation verified
K143033 LacI (Lva-, N terminal deletion) Transformed 25/9
K143034 LipA-EAK16 Fusion Digestion Verified
K143035 LipA-Human Elastin Fusion Transformed 25/9
K143036 Xylose Operon Regulatory Protein Transformed 25/9
K143037 Ytva Blue Light Receptor of B. subtilis Awaiting Ligation
K143038 SacB-EAK16 Fusion Digestion Verified
K143039 SacB-Human Elastin Fusion Awaiting Ligation

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