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BioBricks & Characterisation

Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.

Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.

| Device 1 | Device 2 | Device 3 |
| The System |

Parts Submitted to the Registry

For now, here are the parts we will submit to the Registry. They can also be accessed here.

Production Status

This table shows our progress with each part:

Part Number Part Name Status
K143001 5' AmyE Integration Sequence Midiprepped
K143002 3' AmyE Integration Sequence Midiprepped
K143005 5' EpsE Integration Sequence Awaiting Ligation
K143006 3' EpsE Integration Sequence Awaiting Ligation
K143008 5' PyrD Integration Sequence Awaiting GeneArt delivery
K143009 3' PyrD Integration Sequence Awaiting Ligation
K143010 Promoter ctc for B. subtilis
K143011 Promoter gsiB for B. subtilis
K143012 Promoter veg for B. subtilis
K143013 Promoter 43 for B. subtilis
K143014 Promoter Xyl for B. subtilis
K143015 Promoter hyper-spank for B. subtilis
K143020 GsiB Ribosome Binding Site (RBS) for B. subtilis
K143021 SpoVG Ribosome Binding Site (RBS) for B. subtilis
K143031 Aad9 Spectinomycin Resistance Gene Ligation Failed
K143032 EpsE Molecular Clutch Gene of B. subtilis Midiprepped
K143033 LacI (Lva-, N terminal deletion) PCR verified
K143034 LipA-EAK16 Fusion Midiprepped
K143035 LipA-Human Elastin Fusion Transformed 25/9
K143036 Xylose Operon Regulatory Protein Transformed 29/9
K143037 Ytva Blue Light Receptor of B. subtilis Awaiting Ligation
K143038 SacB-EAK16 Fusion Midiprepped
K143050 P43-gsiB Midiprepped
K143051 P43-spoVG Midiprepped
K143052 Pveg-gsiB Midiprepped
K143053 Pveg-spoVG Midiprepped
K143054 Phyperspank-gsiB Awaiting GeneArt Delivery
K143055 Phyperspank-spoVG Awaiting Ligation
K143056 Pxyl-gsiB Awaiting GeneArt Delivery
K143057 Pxyl-spoVG Awaiting GeneArt Delivery
K143058 Pctc-gsiB Awaiting Ligation
K143059 Pctc-spoVG Awaiting Ligation
K143060 PgsiB-gsiB Midiprepped
K143061 PgsiB-spoVG Midiprepped
K143062 LacI - Terminator Awaiting Components (LacI)
K143063 XylR - Terminator Awaiting Components (XylR)
K143064 CAT - Terminator Awaiting Ligation
K143065 Aad9 - Terminator Awaiting Components (Aad9)

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