IGEM:IMPERIAL/2008/New/Cellulose: Difference between revisions

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|''Acetobacter xylinum'' is a Gram-negative bacterium that produces bacterial cellulose (BC). Central to BC production is cellulose synthase, which synthesizes cellulose from the sugar precursor, UDP-glucose. Cellulose synthase is thought to consist of four subunits known as A,B,C and D. A and B are thought to form a glycosyltransferase that catalyses a β-glycosyltransferase reaction to join two sugar molecules together to progressively create chains of glucose. It is hypothesised that the C subunit forms a pore for the transport of cellulose and D helps to crystallize cellulose into larger fibers. In addition two complementary enzymes appear to be essential for BC production, an endo-beta-1,4-glucanase (Cmcax) and cellulose complementary protein (CcpAx). Cmcax causes hydrolysis of cellulose but has been shown to be essential for BC production, whilst the CcpAx function remains to be determined.
|''Acetobacter xylinum'' is a Gram-negative bacterium that produces bacterial cellulose (BC). Central to BC production is cellulose synthase, which synthesizes cellulose from the sugar precursor, UDP-glucose. Cellulose synthase is thought to consist of four subunits known as A,B,C and D. A and B are thought to form a glycosyltransferase that catalyses a β-glycosyltransferase reaction to join two sugar molecules together to progressively create chains of glucose. It is hypothesised that the C subunit forms a pore for the transport of cellulose and D helps to crystallize cellulose into larger fibers. In addition two complementary enzymes appear to be essential for BC production, an endo-beta-1,4-glucanase (Cmcax) and cellulose complementary protein (CcpAx). Cmcax causes hydrolysis of cellulose but has been shown to be essential for BC production, whilst the CcpAx function remains to be determined.
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|[[Image:CELLULOSE GENES.PNG|300px|center]]
|[[Image:CELLULOSE GENES.PNG|400px|center]]




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Biomaterial
To explore the use of synthetic biology for biomaterial we collaborated with Suzanne Lee, a Senior Research Fellow at St Martins School of Art & Design. She has been focused on the use of a particular biomaterial called bacterial cellulose for the production of clothes. Drying the gel like bacterial cellulose produces a dry sheet that can be cut and manipulated to produce a number clothes from jackets to shoes, see pictures below. From our discussions with Suzanne Lee it became apparent that there were a number of potential advantages for taking a synthetic biology approach to produce biomaterials. This page summaries the use of bacterial cellulose and the potential advantages for a synthetic biology approach.


Structure of Bacterial Cellulose

Although bacterial cellulose has been known and used for many years, its structure was not revealed until X-ray crystallography studies in the 1980's. On the molecular level chains of glucose join together in repeating units that build up microfibers. These microfibers randomly assemble into fibers of ~130nm width. During the drying process of bacterial cellulose, these fibers arrange parallel to each others and form layered sheets. These give the dried cellulose sheets high stability and strength to the cellulose sheets.Traditionally bacterial cellulose has been collected and used in nata-de-coca, an indigenous desert food of Philippines. Recently a number of new applications have been proposed from use in paper production to use as skin patches to assist skin damage.


Genetics of bacterial cellulose
Acetobacter xylinum is a Gram-negative bacterium that produces bacterial cellulose (BC). Central to BC production is cellulose synthase, which synthesizes cellulose from the sugar precursor, UDP-glucose. Cellulose synthase is thought to consist of four subunits known as A,B,C and D. A and B are thought to form a glycosyltransferase that catalyses a β-glycosyltransferase reaction to join two sugar molecules together to progressively create chains of glucose. It is hypothesised that the C subunit forms a pore for the transport of cellulose and D helps to crystallize cellulose into larger fibers. In addition two complementary enzymes appear to be essential for BC production, an endo-beta-1,4-glucanase (Cmcax) and cellulose complementary protein (CcpAx). Cmcax causes hydrolysis of cellulose but has been shown to be essential for BC production, whilst the CcpAx function remains to be determined.


}
The Potential of Synthetic Biology
The potential of synthetic biology for biomaterials lies in controlling synthesis. For example, in our biofabricator subtilis we use a light input to drive the synthesis of biomaterial and halt movement. Using the biofabricator subtilis would allow a greater control of the Microscopic properties (i.e the layout of fibers) and Macroscopic properties (i.e. the overall shape) of a biomaterial. Furthermore, changing the inputs into our biofabricator subtilis could allow more specific control. For example, shaping cellulose around a mold to produce seamless clothes is technically challanging. However, adaption of the biofabrictor subtilis could allow binding and sensing of the mold to allow cellulose synthesis only when the bacteria is bound.



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