IGEM:IMPERIAL/2008/New/Chassis 2: Difference between revisions
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|style="background:#FFFFFF"| '''Single Membrane''' - ''B.subtilis'' is a gram-positive bacterium and contains only a single membrane. This makes ''B.subtilis'' an ideal chassis for secretion of biomaterials. | |style="background:#FFFFFF"| '''Single Membrane''' - ''B. subtilis'' is a gram-positive bacterium and contains only a single membrane. This makes ''B.subtilis'' an ideal chassis for secretion of biomaterials. | ||
|style="background:#FFFFFF"| '''Peptidase activity''' - ''B.subtilis'' express exopeptidases and so there is a risk that our biomaterial may be cleaved up before it can be secreted. | |style="background:#FFFFFF"| '''Peptidase activity''' - ''B. subtilis'' express exopeptidases and so there is a risk that our biomaterial may be cleaved up before it can be secreted. | ||
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|style="background:#FFFFFF"| '''Natural Competency and Integration''' - ''B. subtilis'' has been noted for its ease and efficieny of transformation. In addition, integration of exogenous DNA into the chromosome has been well studied and provides an alternative to using traditional plasmids. | |style="background:#FFFFFF"| '''Natural Competency and Integration''' - ''B. subtilis'' has been noted for its ease and efficieny of transformation. In addition, integration of exogenous DNA into the chromosome has been well studied and provides an alternative to using traditional plasmids. | ||
|style="background:#FFFFFF"| '''Vector Degradation''' - ''B.subtilis'' does not use all the same vectors as ''E.coli''. One reason for this is that ''B.subtilis'' often recognises vectors grown in ''E.coli'' and digests them. | |style="background:#FFFFFF"| '''Vector Degradation''' - ''B. subtilis'' does not use all the same vectors as ''E.coli''. One reason for this is that ''B.subtilis'' often recognises vectors grown in ''E. coli'' as foreign and digests them. Based on experiences of previous iGEM teams, BioBricks also appear to undergo degradation in ''B. subtilis''. | ||
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Revision as of 15:04, 12 September 2008
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