IGEM:IMPERIAL/2008/New/Cloning Strategy: Difference between revisions
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= Cloning Strategy = | = Cloning Strategy = | ||
The Imperial iGEM 2008 team faces the | The Imperial iGEM 2008 team faces the daunting task of working with a chassis that has been rarely used - and never characterised - in the competition so far. While the ''subtilis'' chassis offers us many advantages, working from the ground up like this also presents many challenges. | ||
Our cloning strategy, for instance, is highly complex. In order to building increasingly complicated constructs for our final product, we need to build, test and characterise all the parts and devices leading up to the final systems! The diagram below shows the "critical pathway" for our cloning strategy and as you can see, there are a huge number of closely-linked steps.. | |||
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A summary of constructs that should be produced by the end of each phase for testing is shown below: | |||
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Revision as of 12:30, 11 September 2008
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<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_1">Why B. subtilis?</a>
<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Wet_Lab">Wet Lab Hub</a>
<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Cloning_Strategy">Cloning Strategy</a>
<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols">Experiments & Protocols</a>
<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Major_Results">Experimental Results</a>
<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/BioBricks">BioBricks & Characterisation</a>
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</td><td align="center" width="18%" valign="bottom"><ul id="sddm"><a href="#" onclick="mopen('m3')"
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<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Genetic_Circuit">Genetic Circuits</a>
<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Motility">Motility Analysis</a>
<a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Appendices">Appendices - Code etc.</a>
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</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html> | ||||||||||||||||
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