# IGEM:IMPERIAL/2008/New/Growth Curve

### From OpenWetWare

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- | ==LAG PHASE == | + | ===LAG PHASE === |

During the lag phase, the rate of growth is slow due to two main reasons, ''B. subtilis'' is absorbing nutrients in the medium and the replication machinery is being switched on. The higher the concentration of nutrients in the medium, the faster the rate of bateria growth. | During the lag phase, the rate of growth is slow due to two main reasons, ''B. subtilis'' is absorbing nutrients in the medium and the replication machinery is being switched on. The higher the concentration of nutrients in the medium, the faster the rate of bateria growth. | ||

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As a result, the volume of the bacteria increases, followed by an increases in the number of bacteria. | As a result, the volume of the bacteria increases, followed by an increases in the number of bacteria. | ||

- | == EXPONENTIAL PHASE == | + | === EXPONENTIAL PHASE === |

Both colony number and cell volume increase exponentially during this phase. Our model assumes concentration of the nutrients inside the bacteria is constant. | Both colony number and cell volume increase exponentially during this phase. Our model assumes concentration of the nutrients inside the bacteria is constant. | ||

- | == STATIONARY PHASE == | + | === STATIONARY PHASE === |

The growth of the colony ceases in number and in volume due to a finite concentration of nutrients, hence its does not have a gradient. Other causes may be death and cell division. | The growth of the colony ceases in number and in volume due to a finite concentration of nutrients, hence its does not have a gradient. Other causes may be death and cell division. | ||

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*Time span of lag phase, stationary phase and exponential phase | *Time span of lag phase, stationary phase and exponential phase | ||

*The growth rate | *The growth rate | ||

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== Results == | == Results == | ||

[[Image:Log_graph.tif|thumb|right|200px|Log-Graph used to determine the growth rate]] | [[Image:Log_graph.tif|thumb|right|200px|Log-Graph used to determine the growth rate]] | ||

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CONSTANT: <math>\alpha</math> = 0.64516 | CONSTANT: <math>\alpha</math> = 0.64516 | ||

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## Revision as of 13:38, 27 September 2008

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## DiscussionGrowth Curve |