IGEM:IMPERIAL/2008/New/Growth Curve: Difference between revisions
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During the lag phase, the rate of growth is slow due to two main reasons, ''B. subtilis'' is absorbing nutrients in the medium and the replication machinery is being switched on. The higher the concentration of nutrients in the medium, the faster the rate of bateria growth. | During the lag phase, the rate of growth is slow due to two main reasons, ''B. subtilis'' is absorbing nutrients in the medium and the replication machinery is being switched on. The higher the concentration of nutrients in the medium, the faster the rate of bateria growth. | ||
As a result, the volume of the bacteria increases, followed by an | As a result, the volume of the bacteria increases, followed by an increase in the number of bacteria. | ||
=== EXPONENTIAL PHASE === | === EXPONENTIAL PHASE === | ||
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=== STATIONARY PHASE === | === STATIONARY PHASE === | ||
The growth of the colony ceases in number and in volume due to a finite concentration of nutrients, hence | The growth of the colony ceases in number and in volume due to a finite concentration of nutrients, hence it does not have a gradient. Other causes may be death and cell division. | ||
According to the model, the maximum growth of the bacteria is determined by the concentration of nutrients available initially. | According to the model, the maximum growth of the bacteria is determined by the concentration of nutrients available initially. |
Revision as of 02:38, 30 September 2008
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ResultsThe model for the growth curve was fitted to the experimental results as shown below. The experimental results is depicted by the red curve, while our model is shown by the green curve. The resource curve was also plotted as a function of time and is shown below. Based on our experimental results from the Wet Lab, a log graph was plotted to determine the growth rate. The growth rate was then determined from the gradient of the log graph. This value was included when simulating the growth model using MATLAB.
GROWTH CONSTANT: A = 1.3494 INITIAL NUTRIENT CONCENTRATION: R_0 = 2 HILL COEFFICIENT: n = 1.25 INITIAL OD = 0.4 CONSTANT: [math]\displaystyle{ \alpha }[/math] = 0.64516
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