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{| cellpadding="1" style="background:#2B48B3; border:4px solid #99BBFF; color:#E5EBFF" align="center" width=90%
|<center>Welcome to the Imperial 2008 iGEM project page. It's {{CURRENTDAYNAME}}, {{CURRENTMONTHNAME}} {{CURRENTDAY}} and a great day to read about an awesome iGEM project!</center>
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{{Imperial/Box1|<html><center><img width="350px" src="http://i59.photobucket.com/albums/g305/Timpski/Logo1.png"></center></html>
|For the 2008 iGEM competition, the Imperial College Team aims to develop a genetically-engineered Biofabricator, using the Gram-positive bacterium Bacillus subtilis as our chassis. Our Biofabricator aims to produce self-assembling biomaterials in specified 3D shapes, using light as the trigger.
<br><br>
This is achieved in three stages. First by utilising an endogenous light-sensing mechanism, the bacteria is captured in the desired location using 3D holography. Next bacterial locomotion is suspended in the region of interest using a recently-discovered clutch mechanism. This involves disengaging the flagellum from the motor protein. Finally, when our bacteria are stationary in the correct location, the biomaterial production is triggered. These biomaterials can self-assemble to form a 3D bio-scaffold. Applications of our Biofabricator range from regenerative tissue engineering to Bio-Couture.
<br><br>
[[Image:Imperial_2008_Bioprinter_Cartoon.png |center|600px| Overview of our planned system]]
[[Image:Imperial_2008_Basic_Circuit.jpg |center|Basic Circuit Diagram]]
<br><br>
Please continue on to our project pages - you may want to start with our [[IGEM:IMPERIAL/2008/New/Project| '''>>> Project Specifications >>>''']]}}
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<center>Welcome to the Imperial 2008 iGEM project page. It's {{CURRENTDAYNAME}}, {{CURRENTMONTHNAME}} {{CURRENTDAY}} and a great day to read about an awesome iGEM project!</center>
{{Imperial/Box1||
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The Imperial College Team 2008 has received sponsorship from a number of generous companies. We are grateful for their kind support.
 
<html><center><a href="http://www.bio-rad.com/"><img height="40px" src="http://i59.photobucket.com/albums/g305/Timpski/Biorad.png"></a><a href="http://www.fisher.co.uk/"><img height="50px" src="http://i59.photobucket.com/albums/g305/Timpski/Fisher.png"></a><a href="http://www.geneart.com/"><img height="25px" src="http://i59.photobucket.com/albums/g305/Timpski/Geneart.png"></a><a href="http://www.vwr.com/index.htm"><img height="50px" src="http://i59.photobucket.com/albums/g305/Timpski/VWR.png"></a></center></html>
 
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| For the 2008 iGEM competition, the Imperial College team is working on the foundations for a biofabricator. We are using the Gram-positive ''Bacillus subtilis'' bacterium as our chassis (for a variety of reasons) and hope to exert fine control over its movement via a recently-discovered clutch mechanism [http://www.sciencemag.org/cgi/content/full/sci;320/5883/1636]. Using light as a stimulus to localise the bacteria, we then intend to trigger production and secretion of a self-assembling bio-scaffold material in a set 3D pattern. The project was inspired by 3D printers used in rapid prototyping, and our "blue-sky" aim is to design a full 3D biofabrication machine!
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| [[Image:Imperial_2008_Bioprinter_Cartoon.png |450px| Overview of our planned system]] [[Image:Imperial_2008_Basic_Circuit.jpg | Basic Circuit Diagram]]
| valign="top" |This diagram gives a basic overview of how we intend our system to work. In the starting phase, ''B. subtilis'' are motile and are not producing our desired product - they swim freely in the medium. If we want to construct a bio-scaffold with an "I" shape, we shine light of the correct wavelength (red is used as an arbitrary example here) in the desired shape onto the plate. <br><br>Bacteria within this area will sense that light, and production of a clutch molecule will be triggered. This disengages the flagella from the motor quite quickly, rendering the ''subtilis'' stationary. <br><br>Coupled with the clutch is a gene for expression of peptides for our bio-scaffold material, so they will start producing them when in the area. Should any individuals stray from the correct area, the clutch should disengage and material synthesis should stop. A basic circuit overview of how we hope to achieve this is shown. We had also considered engineering ''B. subtilis'' to release a chemoattractant to bring in "reinforcements" and improve localisation, but this may be beyond the scope of our project.<br><br>3D bio-scaffold materials have many applications in tissue engineering and regenerative medicine. We hope to build up our bio-scaffold material pixel by pixel in the defined area - the basis of our 3D biofabricator.
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[[IGEM:IMPERIAL/2008/Prototype/Test_Page | '''''Test page and storage for random parts - Also see here for intro to editing the Imperial Wiki''''']]
 
[[IGEM:IMPERIAL/2008/Old_Wiki | '''''Revisit the original Wiki layout''''']]
 
Imperial College's iGEM team 2008 would like to thank our sponsors:
<html><center><a href=http://www.bio-rad.com/><img src=http://i59.photobucket.com/albums/g305/Timpski/BioRad.png></a><a href=http://www.fisher.co.uk/><img height=50px src=http://i59.photobucket.com/albums/g305/Timpski/FisherScientific.jpg></a><a href=http://www.geneart.com/><img src=http://i59.photobucket.com/albums/g305/Timpski/GeneArt.gif></a><a href=http://www.vwr.com/index.htm><img height=50px src=http://i59.photobucket.com/albums/g305/Timpski/VWR.jpg></a></center></html>


{{Imperial/EndPage}}
{{Imperial/EndPage|Home|Project}}

Latest revision as of 09:14, 13 October 2008

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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

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Welcome to the Imperial 2008 iGEM project page. It's Saturday, April 20 and a great day to read about an awesome iGEM project!


<html><center><img width="350px" src="http://i59.photobucket.com/albums/g305/Timpski/Logo1.png"></center></html>

For the 2008 iGEM competition, the Imperial College Team aims to develop a genetically-engineered Biofabricator, using the Gram-positive bacterium Bacillus subtilis as our chassis. Our Biofabricator aims to produce self-assembling biomaterials in specified 3D shapes, using light as the trigger.

This is achieved in three stages. First by utilising an endogenous light-sensing mechanism, the bacteria is captured in the desired location using 3D holography. Next bacterial locomotion is suspended in the region of interest using a recently-discovered clutch mechanism. This involves disengaging the flagellum from the motor protein. Finally, when our bacteria are stationary in the correct location, the biomaterial production is triggered. These biomaterials can self-assemble to form a 3D bio-scaffold. Applications of our Biofabricator range from regenerative tissue engineering to Bio-Couture.

Overview of our planned system
Overview of our planned system
Basic Circuit Diagram
Basic Circuit Diagram



Please continue on to our project pages - you may want to start with our >>> Project Specifications >>>


The Imperial College Team 2008 has received sponsorship from a number of generous companies. We are grateful for their kind support. <html><center><a href="http://www.bio-rad.com/"><img height="40px" src="http://i59.photobucket.com/albums/g305/Timpski/Biorad.png"></a><a href="http://www.fisher.co.uk/"><img height="50px" src="http://i59.photobucket.com/albums/g305/Timpski/Fisher.png"></a><a href="http://www.geneart.com/"><img height="25px" src="http://i59.photobucket.com/albums/g305/Timpski/Geneart.png"></a><a href="http://www.vwr.com/index.htm"><img height="50px" src="http://i59.photobucket.com/albums/g305/Timpski/VWR.png"></a></center></html>



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