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{| cellpadding="1" style="background:#2B48B3; border:4px solid #99BBFF; color:#E5EBFF" align="center" width=90%
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|<center>Welcome to the Imperial 2008 iGEM project page. It's {{CURRENTDAYNAME}}, {{CURRENTMONTHNAME}} {{CURRENTDAY}} and a great day to read about an awesome iGEM project!</center>
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<center>Welcome to the Imperial 2008 iGEM project page. It's {{CURRENTDAYNAME}}, {{CURRENTMONTHNAME}} {{CURRENTDAY}} and a great day to read about an awesome iGEM project!</center>
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{{Imperial/Box1|<html><center><img width="350px" src="http://i59.photobucket.com/albums/g305/Timpski/Logo1.png"></center></html>
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|For the 2008 iGEM competition, the Imperial College Team aims to develop a genetically-engineered Biofabricator, using the Gram-positive bacterium Bacillus subtilis as our chassis. Our Biofabricator aims to produce self-assembling biomaterials in specified 3D shapes, using light as the trigger.  
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For the 2008 iGEM competition, the Imperial team is designing a biofabricator using the Gram-positive bacterium ''Bacillus subtilis'' as our chassis. We hope to exert fine control over its movement via a recently-discovered clutch mechanism, using light as our stimulus to localise the bacteria. We then intend to trigger production and secretion of a self-assembling biomaterial in a set 3D pattern.
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3D bio-scaffold materials have many applications in tissue engineering. Our blue-sky aim is to synthesise a precise biofabricator that can accelerate tissue engineering processes, hence making a contribution to the field of regenerative medicine.
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| [[Image:Imperial_2008_Bioprinter_Cartoon.png |380px| Overview of our planned system]]
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[[Image:Imperial_2008_Basic_Circuit.jpg | 380px |Basic Circuit Diagram]]
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This diagram gives an overview of how our system works. Initially, ''B. subtilis'' are motile and are not producing biomaterials. If we want to construct a bio-scaffold with an "I" shape in 3D, we shine a 3D hologram of the correct wavelength (red is used as an arbitrary example here) onto the growth medium.
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Bacteria will sense that light and triggers start to produce a clutch molecule. This disengages the flagella from the motor quite quickly, rendering the ''subtilis'' stationary. Coupled with the clutch is a gene for expression for biomaterial synthesis. Should any individuals stray from the correct area, the clutch should disengage and material synthesis should stop.
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This is achieved in three stages. First by utilising an endogenous light-sensing mechanism, the bacteria is captured in the desired location using 3D holography. Next bacterial locomotion is suspended in the region of interest using a recently-discovered clutch mechanism. This involves disengaging the flagellum from the motor protein. Finally, when our bacteria are stationary in the correct location, the biomaterial production is triggered. These biomaterials can self-assemble to form a 3D bio-scaffold. Applications of our Biofabricator range from regenerative tissue engineering to Bio-Couture.
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We hope to build up our bio-scaffold material pixel by pixel in the defined area - the basis of our 3D biofabrication process.
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[[Image:Imperial_2008_Bioprinter_Cartoon.png |center|600px| Overview of our planned system]]
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[[Image:Imperial_2008_Basic_Circuit.jpg |center|Basic Circuit Diagram]]
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Please continue on to our project pages - you may want to start with our [[IGEM:IMPERIAL/2008/New/Project| '''>>> Project Specifications >>>''']]}}
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The Imperial College Team 2008 has received sponsorship from a number of generous companies. We are grateful for their kind support.
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<html><center><a href="http://www.bio-rad.com/"><img height="40px" src="http://i59.photobucket.com/albums/g305/Timpski/Biorad.png"></a><a href="http://www.fisher.co.uk/"><img height="50px" src="http://i59.photobucket.com/albums/g305/Timpski/Fisher.png"></a><a href="http://www.geneart.com/"><img height="25px" src="http://i59.photobucket.com/albums/g305/Timpski/Geneart.png"></a><a href="http://www.vwr.com/index.htm"><img height="50px" src="http://i59.photobucket.com/albums/g305/Timpski/VWR.png"></a></center></html>
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{{Imperial/EndPage|Home|Project}}
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Please continue on to our project pages - you may want to start with our [[IGEM:IMPERIAL/2008/New/Project| '''>>> Project Specifications >>>''']]...
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Imperial's 2008 iGEM team has received sponsorship from a number of generous companies...
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<html><center><a href=http://www.bio-rad.com/><img src=http://i59.photobucket.com/albums/g305/Timpski/BioRad.png></a><a href=http://www.fisher.co.uk/><img height=50px src=http://i59.photobucket.com/albums/g305/Timpski/FisherScientific.jpg></a><a href=http://www.geneart.com/><img src=http://i59.photobucket.com/albums/g305/Timpski/GeneArt.gif></a><a href=http://www.vwr.com/index.htm><img height=50px src=http://i59.photobucket.com/albums/g305/Timpski/VWR.jpg></a></center></html>
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Current revision






Welcome to the Imperial 2008 iGEM project page. It's Friday, December 26 and a great day to read about an awesome iGEM project!


For the 2008 iGEM competition, the Imperial College Team aims to develop a genetically-engineered Biofabricator, using the Gram-positive bacterium Bacillus subtilis as our chassis. Our Biofabricator aims to produce self-assembling biomaterials in specified 3D shapes, using light as the trigger.

This is achieved in three stages. First by utilising an endogenous light-sensing mechanism, the bacteria is captured in the desired location using 3D holography. Next bacterial locomotion is suspended in the region of interest using a recently-discovered clutch mechanism. This involves disengaging the flagellum from the motor protein. Finally, when our bacteria are stationary in the correct location, the biomaterial production is triggered. These biomaterials can self-assemble to form a 3D bio-scaffold. Applications of our Biofabricator range from regenerative tissue engineering to Bio-Couture.

Overview of our planned system
Basic Circuit Diagram



Please continue on to our project pages - you may want to start with our >>> Project Specifications >>>



The Imperial College Team 2008 has received sponsorship from a number of generous companies. We are grateful for their kind support.



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