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| For the 2008 iGEM competition, the Imperial College team is working on the foundations for a biofabricator. We are using the Gram-positive ''Bacillus subtilis'' bacterium as our chassis and hope to exert fine control over its movement via a recently-discovered clutch mechanism [http://www.sciencemag.org/cgi/content/full/sci;320/5883/1636]. Using light as a stimulus to localise the bacteria, we then intend to trigger production and secretion of a self-assembling bio-scaffold material in a set 3D pattern.
| For the 2008 iGEM competition, the Imperial College team is working on the foundations for a biofabricator. We are using the Gram-positive ''Bacillus subtilis'' bacterium as our chassis and hope to exert fine control over its movement via a recently-discovered clutch mechanism [http://www.sciencemag.org/cgi/content/full/sci;320/5883/1636]. Using light as a stimulus to localise the bacteria, we then intend to trigger production and secretion of a self-assembling bio-scaffold material in a set 3D pattern.
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This diagram gives an overview of how we intend our system to work. In the starting phase, ''B. subtilis'' are motile and are not producing our desired product. If we want to construct a bio-scaffold with an "I" shape, we shine light of the correct wavelength (red is used as an arbitrary example here) in the desired shape onto the plate.
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| [[Image:Imperial_2008_Bioprinter_Cartoon.png |450px| Overview of our planned system]] [[Image:Imperial_2008_Basic_Circuit.jpg | Basic Circuit Diagram]]
| [[Image:Imperial_2008_Bioprinter_Cartoon.png |450px| Overview of our planned system]] [[Image:Imperial_2008_Basic_Circuit.jpg | Basic Circuit Diagram]]
| valign="top" |This diagram gives a basic overview of how we intend our system to work. In the starting phase, ''B. subtilis'' are motile and are not producing our desired product. If we want to construct a bio-scaffold with an "I" shape, we shine light of the correct wavelength (red is used as an arbitrary example here) in the desired shape onto the plate.
| valign="top" |
 
<br><br>Bacteria will sense that light, and production of a clutch molecule will be triggered. This disengages the flagella from the motor quite quickly, rendering the ''subtilis'' stationary. <br><br>Coupled with the clutch is a gene for expression for biomaterial synthesis. Should any individuals stray from the correct area, the clutch should disengage and material synthesis should stop.
<br><br>Bacteria will sense that light, and production of a clutch molecule will be triggered. This disengages the flagella from the motor quite quickly, rendering the ''subtilis'' stationary. <br><br>Coupled with the clutch is a gene for expression for biomaterial synthesis. Should any individuals stray from the correct area, the clutch should disengage and material synthesis should stop.


Revision as of 06:39, 11 September 2008

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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

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Welcome to the Imperial 2008 iGEM project page. It's Friday, April 19 and a great day to read about an awesome iGEM project!


<html><center><embed width=640px src="http://openwetware.org/images/5/52/Imperial_2008_Movie.mov"></embed></center></html>


For the 2008 iGEM competition, the Imperial College team is working on the foundations for a biofabricator. We are using the Gram-positive Bacillus subtilis bacterium as our chassis and hope to exert fine control over its movement via a recently-discovered clutch mechanism [1]. Using light as a stimulus to localise the bacteria, we then intend to trigger production and secretion of a self-assembling bio-scaffold material in a set 3D pattern.



This diagram gives an overview of how we intend our system to work. In the starting phase, B. subtilis are motile and are not producing our desired product. If we want to construct a bio-scaffold with an "I" shape, we shine light of the correct wavelength (red is used as an arbitrary example here) in the desired shape onto the plate.



Overview of our planned system Basic Circuit Diagram



Bacteria will sense that light, and production of a clutch molecule will be triggered. This disengages the flagella from the motor quite quickly, rendering the subtilis stationary.

Coupled with the clutch is a gene for expression for biomaterial synthesis. Should any individuals stray from the correct area, the clutch should disengage and material synthesis should stop.



3D bio-scaffold materials have many applications in tissue engineering and regenerative medicine. We hope to build up our bio-scaffold material pixel by pixel in the defined area - the basis of our 3D biofabrication process.


Test page and storage for random parts - Also see here for intro to editing the Imperial Wiki

Revisit the original Wiki layout

Imperial College's iGEM team 2008 would like to thank our sponsors: <html><center><a href=http://www.bio-rad.com/><img src=http://i59.photobucket.com/albums/g305/Timpski/BioRad.png></a><a href=http://www.fisher.co.uk/><img height=50px src=http://i59.photobucket.com/albums/g305/Timpski/FisherScientific.jpg></a><a href=http://www.geneart.com/><img src=http://i59.photobucket.com/albums/g305/Timpski/GeneArt.gif></a><a href=http://www.vwr.com/index.htm><img height=50px src=http://i59.photobucket.com/albums/g305/Timpski/VWR.jpg></a></center></html>


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