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Calibration Curve - O.D.600 vs Colony Forming Units
In order for us to convert the non standardised fluorescence data from the promoter characterisation into the standardized units of rate of GFPmut3b Synthesis per second per colony forming unit, we have constructed a calibration curve to convert O.D.600 to colony forming units. Using this calibration curve fluorescence data can be normalised based upon the number of colony forming units which approximates to the number of cells.
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Transformation Protocol 2
Brief Descrption of protocol
Results |