In designing our Biofabricator Subtilis, we followed the engineering approach for synthetic biology.
We started with a set of basic specifications our project must meet in order to fulfill our aim of designing a genetically-engineering biofabricator. Since there are three main parts to our project, our specifications can be broadly classified into three categories - light sensing, motility control and biomaterial synthesis.
The photoreceptor must have a short response time, preferably under one minute.
The light sensing pathway must be able to be coupled to an inducible promoter, to allow control of our downstream systems (motility control and biomaterial production).
The photoreceptor must be compatible with the chassis.
The pathway must not give rise to negative secondary effects such as interference with native pathways.
Quick and effective control of bacteria motility - we should be able to stop bacteria locomotion efficiently.
The mechanism for motility control should be reversible - bacteria should be able to regain locomotion after a certain recovery period, but this is not essential.
Bacteria should be able to secrete large amounts of a self-assembling biomaterial into the growth medium.
Biomaterial must be small enough to facilitate its secretion by the chassis.
Biomaterial should be compatible with tissue engineering or regenerative medicine.
For more information on how B. subtilis meets our specifications and why we have embarked on the ambitious route of using it as our chassis, please refer to the next page. >>> Why B. subtilis? >>>