IGEM:IMPERIAL/2008/Projects/B. subtilis Chassis: Difference between revisions
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[[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=557300| pC194]] | [[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=557300| pC194]] | ||
Given the activity of B. subtilis degrading E. coli derived plasmids, amybe it would be an idea to utilise PCR for plasmid production and selection of transformants in B. subtilis to by-pass this issue? | |||
==Promoters== | ==Promoters== | ||
Constitutive promoters | |||
Use of basal transcription promoters, potentially the promoter for one or various rRNAs | |||
All non-constiutive promoters should remain functional in B. subtilis though basal leaky (basal) transcription rate will however be different | |||
==RBSs?== | ==RBSs?== | ||
Needs research... |
Revision as of 16:54, 9 July 2008
To B.subtilis or not to B. subtilis, that is THE question
Vector
[pC194]
[pC194]
Given the activity of B. subtilis degrading E. coli derived plasmids, amybe it would be an idea to utilise PCR for plasmid production and selection of transformants in B. subtilis to by-pass this issue?
Promoters
Constitutive promoters
Use of basal transcription promoters, potentially the promoter for one or various rRNAs
All non-constiutive promoters should remain functional in B. subtilis though basal leaky (basal) transcription rate will however be different
RBSs?
Needs research...