IGEM:IMPERIAL/2008/Projects/B. subtilis Chassis

To B.subtilis or not to B. subtilis, that is THE question

Vector

[pC194]

[pC194]

Given the activity of B. subtilis degrading E. coli derived plasmids, amybe it would be an idea to utilise PCR for plasmid production and selection of transformants in B. subtilis to by-pass this issue?

Promoters

Constitutive promoters

Use of basal transcription promoters, potentially the promoter for one or various rRNAs

All non-constiutive promoters should remain functional in B. subtilis though basal leaky (basal) transcription rate will however be different

RBSs?

Needs research...