IGEM:IMPERIAL/2008/Prototype: Difference between revisions
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| [[Image:Imperial_2008_Bioprinter_Cartoon.png |450px| Overview of our planned system]] [[Image:Imperial_2008_Basic_Circuit.jpg | Basic Circuit Diagram]] | | [[Image:Imperial_2008_Bioprinter_Cartoon.png |450px| Overview of our planned system]] [[Image:Imperial_2008_Basic_Circuit.jpg | Basic Circuit Diagram]] | ||
| valign="top" |This diagram gives a basic overview of how we intend our system to work. In the starting phase, ''B. subtilis'' are motile and are not producing our desired product - they swim freely in the medium. If we want to construct a bio-scaffold with an "I" shape, we shine light of the correct wavelength (red is used as an arbitrary example here) in the desired shape onto the plate. <br><br>Bacteria within this area will sense that light, and production of a clutch molecule will be triggered. This disengages the flagella from the motor quite quickly, rendering the ''subtilis'' stationary. <br><br>Coupled with the clutch is a gene for expression of peptides for our bio-scaffold material, so they will start producing them when in the area. Should any individuals stray from the correct area, the clutch should disengage and material synthesis should stop. A basic circuit overview of how we hope to achieve this is shown. We had also considered engineering ''B. subtilis'' to release a chemoattractant to bring in "reinforcements" and improve localisation, but this may be beyond the scope of our project.<br><br>3D bio-scaffold materials have many applications in tissue engineering and regenerative medicine. We hope to build up our bio-scaffold material pixel by pixel in the defined area - the basis of our 3D | | valign="top" |This diagram gives a basic overview of how we intend our system to work. In the starting phase, ''B. subtilis'' are motile and are not producing our desired product - they swim freely in the medium. If we want to construct a bio-scaffold with an "I" shape, we shine light of the correct wavelength (red is used as an arbitrary example here) in the desired shape onto the plate. <br><br>Bacteria within this area will sense that light, and production of a clutch molecule will be triggered. This disengages the flagella from the motor quite quickly, rendering the ''subtilis'' stationary. <br><br>Coupled with the clutch is a gene for expression of peptides for our bio-scaffold material, so they will start producing them when in the area. Should any individuals stray from the correct area, the clutch should disengage and material synthesis should stop. A basic circuit overview of how we hope to achieve this is shown. We had also considered engineering ''B. subtilis'' to release a chemoattractant to bring in "reinforcements" and improve localisation, but this may be beyond the scope of our project.<br><br>3D bio-scaffold materials have many applications in tissue engineering and regenerative medicine. We hope to build up our bio-scaffold material pixel by pixel in the defined area - the basis of our 3D biofabricator. | ||
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Revision as of 09:23, 5 September 2008
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| <html><a href=http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype><img width=50px src=http://openwetware.org/images/f/f2/Imperial_2008_Logo.png></img</a></html> | Home | The Project | B.subtilis Chassis | Wet Lab | Dry Lab | Notebook |
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| For the 2008 iGEM competition, the Imperial College team is working on the foundations for a biofabricator. We are using the Gram-positive Bacillus subtilis bacterium as our chassis (for a variety of reasons) and hope to exert fine control over its movement via a recently-discovered clutch mechanism [1]. Using light as a stimulus to localise the bacteria, we then intend to trigger production and secretion of a self-assembling bio-scaffold material in a set pattern. The project was inspired by 3D fabricators used in production of prototypes for manufacturing, and our "blue-sky" aim is to design a 3D biofabricator! |
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This diagram gives a basic overview of how we intend our system to work. In the starting phase, B. subtilis are motile and are not producing our desired product - they swim freely in the medium. If we want to construct a bio-scaffold with an "I" shape, we shine light of the correct wavelength (red is used as an arbitrary example here) in the desired shape onto the plate. Bacteria within this area will sense that light, and production of a clutch molecule will be triggered. This disengages the flagella from the motor quite quickly, rendering the subtilis stationary. Coupled with the clutch is a gene for expression of peptides for our bio-scaffold material, so they will start producing them when in the area. Should any individuals stray from the correct area, the clutch should disengage and material synthesis should stop. A basic circuit overview of how we hope to achieve this is shown. We had also considered engineering B. subtilis to release a chemoattractant to bring in "reinforcements" and improve localisation, but this may be beyond the scope of our project. 3D bio-scaffold materials have many applications in tissue engineering and regenerative medicine. We hope to build up our bio-scaffold material pixel by pixel in the defined area - the basis of our 3D biofabricator. |
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Imperial College's iGEM team 2008 would like to thank our sponsors: <html><center><a href=http://www.bio-rad.com/><img src=http://i59.photobucket.com/albums/g305/Timpski/BioRad.png></a><a href=http://www.fisher.co.uk/><img height=50px src=http://i59.photobucket.com/albums/g305/Timpski/FisherScientific.jpg></a><a href=http://www.geneart.com/><img src=http://i59.photobucket.com/albums/g305/Timpski/GeneArt.gif></a><a href=http://www.vwr.com/index.htm><img height=50px src=http://i59.photobucket.com/albums/g305/Timpski/VWR.jpg></a></center></html>
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