IGEM:IMPERIAL/2008/Prototype/Drylab/Data Analysis/Track Analysis: Difference between revisions
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=Validation= | = Validation = | ||
A synthetic video of five cells with changing shape was used in the evaluation of tracking software. Each cell was tracked using respective tracking software, and their coordinates in pixels were obtained. | A synthetic video of five cells with changing shape was used in the evaluation of tracking software. Each cell was tracked using respective tracking software, and their coordinates in pixels were obtained. | ||
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The errors associated with manual tracking and Volocity software are shown below. All errors are given in terms of pixels. | The errors associated with manual tracking and Volocity software are shown below. All errors are given in terms of pixels. | ||
==Manual Tracking== | == Manual Tracking == | ||
{| border="1" cellpadding="5" cellspacing="0" align="center" | {| border="1" cellpadding="5" cellspacing="0" align="center" | ||
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|} | |} | ||
==Volocity== | == Volocity == | ||
{| border="1" cellpadding="5" cellspacing="0" align="center" | {| border="1" cellpadding="5" cellspacing="0" align="center" | ||
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=Evaluation= | =Evaluation= | ||
==Volocity== | == Volocity == | ||
===Pros=== | === Pros === | ||
*Lower error in terms of tracking centroid than other tracking software. | *Lower error in terms of tracking centroid than other tracking software. | ||
*Less time consuming. | *Less time consuming. | ||
===Cons=== | === Cons === | ||
*Failure to track cells continuously over a long number of frames > 70 | *Failure to track cells continuously over a long number of frames > 70 | ||
*Baised to cells which run, rather than tumble | *Baised to cells which run, rather than tumble | ||
==Manual Tracking== | == Manual Tracking == | ||
===Pros=== | === Pros === | ||
*Able to discern cells visually | *Able to discern cells visually | ||
*Able track over larger number of frames | *Able to track over larger number of frames | ||
===Cons=== | === Cons === | ||
*Time consuming | *Time consuming | ||
*Higher error in terms of tracking centroid as compared to tracking software. | *Higher error in terms of tracking centroid as compared to tracking software. | ||
==Conclusion== | == Conclusion == | ||
*Manual tracking will be used to track ''B.Subtilis''. | *Manual tracking will be used to track ''B.Subtilis''. | ||
{{Imperial/EndPage}} | {{Imperial/EndPage}} | ||
Latest revision as of 13:06, 16 September 2008
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The errors associated with manual tracking and Volocity software are shown below. All errors are given in terms of pixels. Manual Tracking
Volocity
EvaluationVolocityPros
Cons
Manual TrackingPros
Cons
Conclusion
|
