IGEM:IMPERIAL/2008/Prototype/Wetlab/parts/Composite

Composite Parts

Seen as how the wiki may soon cause errors due to the size of the previous page (and the affect it was having on us), here is the new parts page, specifically for composite parts...

We'll start looking at these soon and I'll get back to people after the weekend

Expression Related

P43-gsiB

Name: Promoter 43 and RBS gsiB for B. subtilis

Code: K143050

Long: Constitutive promoter 43(<bbpart>BBa_K143013</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from B. subtilis.

P43-gsiB can be used in the context of a Polymerase per second (PoPS) output generator

Design: The sequence of P43 and RBS-gsiB were obtained from papers[1, 2] and the sequence synthesised by GeneArt

Source: P43-gsiB was synthesised by GeneArt

References

1. Zhang XZ, Cui ZL, Hong Q, and Li SP. High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis WB800. Appl Environ Microbiol. 2005 Jul;71(7):4101-3. DOI:10.1128/AEM.71.7.4101-4103.2005 |
2. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

P43-spoVG

Name:

Code: K143051

Long:

Design:

Source:

Pveg-gsiB

Name:

Code: K143052

Long:

Design:

Source:

Pveg-spoVG

Name:

Code: K143053

Long:

Design:

Source:

Phyper-spank-gsiB

Name:

Code: K143054

Long:

Design:

Source:

Phyper-spank-spoVG

Name:

Code: K143055

Long:

Design:

Source:

Pxyl-gsiB

Name:

Code: K143056

Long:

Design:

Source:

Pxyl-spoVG

Name:

Code: K143057

Long:

Design:

Source:

Pctc-gsiB

Name:

Code: K143058

Long:

Design:

Source:

Pctc-spoVG

Name:

Code: K143059

Long:

Design:

Source:

PgsiB-gsiB

Name:

Code: K143060

Long:

Design:

Source:

PgsiB-spoVG

Name:

Code: K143061

Long:

Design:

Source:

LacI-Terminator

Name:

Code: K143064

Long:

Design:

Source:

XylR-Terminator

Name:

Code: K143065

Long:

Design:

Source:

Codes and associated parts

Note

• Aad9 is the Spectinomycin resistance gene
• RI - Resistance Integration Brick, P - Promoter, Pi - chemically inducible promoter, Pl - light inducible promoter, Bs - B.subtilis, PTC - Promoter Testing Construct, Rep - Repressor protein

Code	Part	Code	Part	Code	Part	Code	Part	Code	Part
K143050	P43-gsiB	K143051	P43-spoVG	K143052	Pveg-gsiB	K143053	Pveg-spoVG	K143054	Phyperspank-gsiB
K143055	Phyper-spank-spoVG	K143056	Pxyl-gsiB	K143057	Pxyl-spoVG	K143058	Pctc-gsiB	K143059	Pctc-spoVG
K143060	PgsiB-gsiB	K143061	PgsiB-spoVG	K143062	CAT - Terminator	K143063	Aad9 - Terminator	K143064	LacI - Terminator
K143065	XylR - Terminator	K143066	Int Open	K143067	RI Brick	K143068	RI-Rep-Pi Brick	K143069	RI-Ytva-Pl Brick
K143070	Bs-PTC	K143071	Int Close	K143072		K143073		K143074
K143075		K143076		K143077		K143078		K143079
K143080		K143081		K143082		K143083		K143084
K143085		K143086		K143087		K143088		K143089