IGEM:IMPERIAL/2008/Prototype/Wetlab/parts/Composite

Composite Parts

Seen as how the wiki may soon cause errors due to the size of the previous page (and the affect it was having on us), here is the new parts page, specifically for composite parts...

We'll start looking at these soon and I'll get back to people after the weekend

Expression Related

P43-gsiB (done)

Name: Promoter 43 and RBS gsiB for B. subtilis

Code: K143050

Long: Constitutive promoter 43(<bbpart>BBa_K143013</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from B. subtilis.

P43-gsiB can be used in the context of a Ribosomes per second (PoPS) output generator

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of P43 and RBS-gsiB were obtained from papers[1, 2] and the sequence synthesised by GeneArt

Source: P43-gsiB was synthesised by GeneArt

References

1. Zhang XZ, Cui ZL, Hong Q, and Li SP. High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis WB800. Appl Environ Microbiol. 2005 Jul;71(7):4101-3. DOI:10.1128/AEM.71.7.4101-4103.2005 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

2. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

P43-spoVG (done)

Name: Promoter 43 and RBS spoVG for B. subtilis

Code: K143051

Long: Constitutive promoter 43(<bbpart>BBa_K143013</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from B. subtilis.

P43-spoVG can be used in the context of a Ribosomes per seconds (PoPS) output generator

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of P43 and RBS-spoVG were obtained from papers[1, 2] and the sequence synthesised by GeneArt

Source: P43-spoVG was synthesised by GeneArt

References

1. Zhang XZ, Cui ZL, Hong Q, and Li SP. High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis WB800. Appl Environ Microbiol. 2005 Jul;71(7):4101-3. DOI:10.1128/AEM.71.7.4101-4103.2005 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

2. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

Pveg-gsiB (done)

Name: Promoter Pveg and RBS gsiB for B. subtilis

Code: K143052

Long: Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from B. subtilis.

Pveg-gsiB can be used in the context of a Ribosomes per second (RiPS) output generator

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of Pveg was obtained from the DBTBS[1] and RBS-gsiB were obtained from papers[2] and the sequence synthesised by GeneArt

Source: Pveg-gsiB was synthesised by GeneArt

References

1. Sierro N, Makita Y, de Hoon M, and Nakai K. DBTBS: a database of transcriptional regulation in Bacillus subtilis containing upstream intergenic conservation information. Nucleic Acids Res. 2008 Jan;36(Database issue):D93-6. DOI:10.1093/nar/gkm910 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

2. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

Pveg-spoVG (Done)

Name: Promoter Pveg and RBS spoVG for B. subtilis

Code: K143053

Long: Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from B. subtilis.

Pveg-spoVG can be used in the context of a Ribosomes per second (RiPS) output generator

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of Pveg was obtained from the DBTBS[1] and RBS-spoVG were obtained from papers[2] and the sequence synthesised by GeneArt

Source: Pveg-spoVG was synthesised by GeneArt

References

1. Sierro N, Makita Y, de Hoon M, and Nakai K. DBTBS: a database of transcriptional regulation in Bacillus subtilis containing upstream intergenic conservation information. Nucleic Acids Res. 2008 Jan;36(Database issue):D93-6. DOI:10.1093/nar/gkm910 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

2. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

Phyper-spank-gsiB (Done)

Name: Promoter hyper-spank and RBS gsiB for B. subtilis

Code: K143054

Long: Inducible promoter hyper-spank(<bbpart>BBa_K143015</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from B. subtilis.

Phyperspank-gsiB can be used to take an input of IPTG and give a Ribosomes per second (RiPS) output generator.

IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, (<bbpart>BBa_K143035</bbpart>). This means that LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of promoter hyperspank was obtained from the B. subtilis integration vector pDR111 and RBS-gsiB were obtained from papers[1] and the sequence synthesised by GeneArt

Source: Phyperspank-gsiB was synthesised by GeneArt

References

1. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

Phyper-spank-spoVG (Done)

Name: Promoter hyper-spank and RBS spoVG for B. subtilis

Code: K143055

Long: Inducible promoter hyper-spank(<bbpart>BBa_K143015</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from B. subtilis.

Phyperspank-spoVG can be used to take an input of IPTG and give a Ribosomes per second (RiPS) output generator.

IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, (<bbpart>BBa_K143033</bbpart>). This means that LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of promoter hyperspank was obtained from the B. subtilis integration vector pDR111 and RBS-spoVG were obtained from papers[1] and the sequence synthesised by GeneArt

Source: Phyperspank-spoVG was synthesised by GeneArt

References

1. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

Pxyl-gsiB (Done)

Name: Promoter xyl and RBS gsiB for B. subtilis

Code: K143056

Long: Inducible promoter xyl(<bbpart>BBa_K143014</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from B. subtilis.

Pxyl-gsiB can be used to take an input of xylose and give a Ribosomes per second (RiPS) output generator.

Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator XylR, (<bbpart>BBa_K143036</bbpart>). This means that XylR must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by B. subtilis but should be upregulated to increase efficiency.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of promoter xyl and RBS-gsiB were obtained from papers[1, 2, 3] and the sequence synthesised by GeneArt

Source: Pxyl-gsiB was synthesised by GeneArt

References

1. Kim L, Mogk A, and Schumann W. A xylose-inducible Bacillus subtilis integration vector and its application. Gene. 1996 Nov 28;181(1-2):71-6. DOI:10.1016/s0378-1119(96)00466-0 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

2. Härtl B, Wehrl W, Wiegert T, Homuth G, and Schumann W. Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes. J Bacteriol. 2001 Apr;183(8):2696-9. DOI:10.1128/JB.183.8.2696-2699.2001 |
3. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

Pxyl-spoVG (Done)

Name: Promoter xyl and RBS spoVG for B subtilis

Code: K143057

Long:Inducible promoter xyl(<bbpart>BBa_K143014</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from B. subtilis.

Pxyl-spoVG can be used to take an input of xylose and give a Ribosomes per second (RiPS) output generator.

Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator XylR, (<bbpart>BBa_K143036</bbpart>). This means that XylR must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by B. subtilis but should be upregulated to increase efficiency.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of promoter xyl and RBS-spoVG were obtained from papers[1, 2, 3] and the sequence synthesised by GeneArt

Source: Pxyl-spoVG was synthesised by GeneArt

References

1. Kim L, Mogk A, and Schumann W. A xylose-inducible Bacillus subtilis integration vector and its application. Gene. 1996 Nov 28;181(1-2):71-6. DOI:10.1016/s0378-1119(96)00466-0 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

2. Härtl B, Wehrl W, Wiegert T, Homuth G, and Schumann W. Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes. J Bacteriol. 2001 Apr;183(8):2696-9. DOI:10.1128/JB.183.8.2696-2699.2001 |
3. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

Pctc-gsiB (Done)

Name Promoter ctc and RBS gsiB for B. subtilis

Code: K143058

Long: Sigma B dependant promoter ctc(<bbpart>BBa_K143010</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from B. subtilis.

In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol[1]. The promoter ctc has been used previously as a read out for the activation of sigma factor B [2].

Pctc has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA (<bbpart>BBa_K143037</bbpart>) is required.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of promoter ctc was obtained from the B. subtilis genome and published papers[1, 2]. RBS-gsiB were obtained from papers[3] and the sequence synthesised by GeneArt

Source: Pctc-gsiB was synthesised by GeneArt

References

1. Igo MM and Losick R. Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis. J Mol Biol. 1986 Oct 20;191(4):615-24. DOI:10.1016/0022-2836(86)90449-3 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

2. Suzuki N, Takaya N, Hoshino T, and Nakamura A. Enhancement of a sigma(B)-dependent stress response in Bacillus subtilis by light via YtvA photoreceptor. J Gen Appl Microbiol. 2007 Apr;53(2):81-8. DOI:10.2323/jgam.53.81 |
3. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

Pctc-spoVG (Done)

Name: Promoter ctc and RBS spoVG for B. subtilis

Code: K143059

Long: Sigma B dependant promoter ctc(<bbpart>BBa_K143010</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from B. subtilis.

In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol[1]. The promoter ctc has been used previously as a read out for the activation of sigma factor B [2].

Pctc has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA (<bbpart>BBa_K143037</bbpart>) is required.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of promoter ctc was obtained from the B. subtilis genome and published papers[1, 2]. RBS-spoVG were obtained from papers[3] and the sequence synthesised by GeneArt

Source: Pctc-spoVG was synthesised by GeneArt

References

1. Igo MM and Losick R. Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis. J Mol Biol. 1986 Oct 20;191(4):615-24. DOI:10.1016/0022-2836(86)90449-3 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

2. Suzuki N, Takaya N, Hoshino T, and Nakamura A. Enhancement of a sigma(B)-dependent stress response in Bacillus subtilis by light via YtvA photoreceptor. J Gen Appl Microbiol. 2007 Apr;53(2):81-8. DOI:10.2323/jgam.53.81 |
3. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

PgsiB-gsiB (Done)

Name: Promoter gsiB and RBS gsiB for B. subtilis

Code: K143060

Long: Sigma B dependant promoter gsiB(<bbpart>BBa_K143011</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from B. subtilis.

In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol[1]. The promoter ctc has been used previously as a read out for the activation of sigma factor B [2].

PgsiB has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA (<bbpart>BBa_K143037</bbpart>) is required.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of promoter gsiB was obtained from the B. subtilis genome and published papers[1, 2]. RBS-gsiB were obtained from papers[3] and the sequence synthesised by GeneArt

Source: PgsiB-gsiB was synthesised by GeneArt

References

1. Igo MM and Losick R. Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis. J Mol Biol. 1986 Oct 20;191(4):615-24. DOI:10.1016/0022-2836(86)90449-3 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

2. Suzuki N, Takaya N, Hoshino T, and Nakamura A. Enhancement of a sigma(B)-dependent stress response in Bacillus subtilis by light via YtvA photoreceptor. J Gen Appl Microbiol. 2007 Apr;53(2):81-8. DOI:10.2323/jgam.53.81 |
3. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

PgsiB-spoVG (Done)

Name: Promoter gsiB and RBS spoVG for B. subtilis

Code: K143061

Long: Sigma B dependant promoter gsiB(<bbpart>BBa_K143011</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from B. subtilis.

In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol[1]. The promoter ctc has been used previously as a read out for the activation of sigma factor B [2].

PgsiB has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA (<bbpart>BBa_K143037</bbpart>) is required.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix[1]. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Design: The sequence of promoter gsiB was obtained from the B. subtilis genome and published papers[1, 2]. RBS-spoVG were obtained from papers[3] and the sequence synthesised by GeneArt

Source: Pctc-spoVG was synthesised by GeneArt

References

1. Igo MM and Losick R. Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis. J Mol Biol. 1986 Oct 20;191(4):615-24. DOI:10.1016/0022-2836(86)90449-3 |

1. http://partsregistry.org/Assembly:RBS-CDS_issues

2. Suzuki N, Takaya N, Hoshino T, and Nakamura A. Enhancement of a sigma(B)-dependent stress response in Bacillus subtilis by light via YtvA photoreceptor. J Gen Appl Microbiol. 2007 Apr;53(2):81-8. DOI:10.2323/jgam.53.81 |
3. Jürgen B, Schweder T, and Hecker M. The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site. Mol Gen Genet. 1998 Jun;258(5):538-45. DOI:10.1007/s004380050765 |

LacI-Terminator (Done)

Name: LacI repressor protein - Terminator

Code: K143062

Long: LacI transcriptional repressor protein (<bbpart>BBa_K143033</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>.

The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with B. subtilis.

LacI can be used in conjunction with the lac operon promoter (<bbpart>BBa_K143015</bbpart>), where the LacI will act as a receiver for an IPTG input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.

Design: LacI was identified from the pDR111 B. subtilis integration vector. The double terminator is the most commonly used registry terminator.

Source: LacI was produced by PCR cloning using Pfu form the B. subtilis integration vector and cloned into a BioBrick with the registry double terminator

XylR-Terminator (Done)

Name: Xylose operon repressor protein - Terminator

Code: K143063

Long: Xylose operon repressor protein(XylR)(<bbpart>BBa_K143036</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>.

XylR can be used in conjunction with the xylose operon promoter (<bbpart>BBa_K143014</bbpart>), where the XylR will act as a receiver for a xylose input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the XylR to be easily incorporated into a closed transcriptional unit.

Design: XylR was identified from the B. subtilis chromosome. The double terminator is the most commonly used registry terminator.

Source: XylR was produced by PCR cloning using Pfu form the B. subtilis chromosome and cloned into a BioBrick with the registry double terminator

YtvA-Terminator (To Do)

Name: YtvA light receptor Protein - Terminator

Code: K143066

Long: YtvA light receptor protein (YtvA)(<bbpart>BBa_K143037</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

YtvA can be used in conjunction with the sigma factor B specific promoter (<bbpart>BBa_K143010</bbpart> or <bbpart>BBa_K143011</bbpart>), where the YtvA will act as a receiver for a blue light input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the YtvA to be easily incorporated into a closed transcriptional unit.

Design: YtvA was identified from the B. subtilis chromosome. The double terminator is the most commonly used registry terminator.

Source: YtvA was synthesised by GeneArt and cloned into a BioBrick with the registry double terminator

Antibiotic Related

Chloraphemicol Resistance Protein - Terminator (Done)

Name: Chloraphemicol resistance protein - Terminator

Code: K143064

Long: Chloraphemicol acetyltransferase protein(<bbpart>BBa_J31005</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Chloraphemicol acetyltransferase confers resistance to Chloraphemicol

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the CAT to be incorporated into a closed transcriptional unit.

Design: Chloraphemicol acetyltransferase is an exisiting registry protein. The double terminator is the most commonly used registry termiantor

Source: The Chloraphemicol acetyltransferase and double terminator were taken both taken from the registry.

Spectinomycin Resistance Protein (Aad9) - Terminator (Done)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143065

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

Integration Related

AmyE integratable PoPS generator (P43-gsiB) (To Do)

Name: AmyE integratable PoPS generator (P43-gsiB)

Code: K143067

Long: AmyE 5' Integration sequence(<bbpart>BBa_K143001</bbpart>) coupled to the PoPS generator P43-gsiB (<bbpart>BBa_K143050</bbpart>).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence(<bbpart>BBa_K143002</bbpart>) is cloned onto the 3' end of the construct.

The P43-gsiB promoter and RBS for B. subtilis constitutively generate a PoPS output.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

AmyE integratable PoPS generator (P43-spoVG) (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143068

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

AmyE integratable PoPS generator (Pveg-gsiB) (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143069

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

AmyE integratable PoPS generator (Pveg-spoVG) (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143070

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

AmyE integratable PoPS generator (P43-gsiB) (with CmR) (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143071

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

AmyE integratable PoPS generator (P43-spoVG) (with CmR) (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143072

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

AmyE integratable PoPS generator (Pveg-gsiB) (with CmR) (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143073

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

AmyE integratable PoPS generator (Pveg-spoVG) (with CmR) (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143074

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

B. subtilis AmyE locus GFP promoter and RBS tester (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143075

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

B. subtilis AmyE locus RFP promoter and RBS tester (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143076

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

P43-gsiB GFP expression construct (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143077

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

P43-spoVG GFP expression construct (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143078

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

Pveg-spoVG GFP expression construct (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143079

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

P43-gsiB RFP expression construct (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143080

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

P43-spoVG RFP expression construct (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143081

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

Pveg-spoVG RFP expression construct (To Do)

Name: Spectinomycin Resistance Protein (Aad9) - Terminator

Code: K143082

Long: Aad9 spectinomycin resistance protein(<bbpart>BBa_</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>).

Aad9 confers resistance to spectinomycin.

The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>.

The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.

Design: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source: Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry

Codes and associated parts

Note

• Aad9 is the Spectinomycin resistance gene
• RI - Resistance Integration Brick, P - Promoter, Pi - chemically inducible promoter, Pl - light inducible promoter, Bs - B.subtilis, PTC - Promoter Testing Construct, Rep - Repressor protein

Code	Part	Code	Part	Code	Part	Code	Part	Code	Part
K143050	P43-gsiB	K143051	P43-spoVG	K143052	Pveg-gsiB	K143053	Pveg-spoVG	K143054	Phyperspank-gsiB
K143055	Phyper-spank-spoVG	K143056	Pxyl-gsiB	K143057	Pxyl-spoVG	K143058	Pctc-gsiB	K143059	Pctc-spoVG
K143060	PgsiB-gsiB	K143061	PgsiB-spoVG	K143062	LacI - Terminator	K143063	XylR - Terminator	K143064	CAT - Terminator
K143065	Aad9 - Terminator	K143066	Ytva - Terminator	K143067	5' 9	K143068	5' 10	K143069	5' 11
K143070	5' 12	K143071	RI Brick 9 tail	K143072	RI Brick 10 tail	K143073	RI Brick 11 tail	K143074	RI Brick 12 tail
K143075	GFP - Terminator 3'	K143076	RFP - Terminator 3'	K143077	P43-gsiB GFP expression construct	K143078	P43-spoVG GFP expression construct	K143079	Pveg-spoVG GFP expression construct
K143080	P43-gsiB RFP expression construct	K143081	P43-spoVG RFP expression construct	K143082	Pveg-spoVG RFP expression construct	K143083		K143084
K143085		K143086		K143087		K143088		K143089