IGEM:IMPERIAL/2008/Sequence: Difference between revisions
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| 2.Promoter - σB || PgsiB || [http://openwetware.org/wiki/Image:PgsiB.doc PgsiB] <br> [http://openwetware.org/wiki/Image:PgsiB_Biobrick.doc Biobrick] || [http://www.ncbi.nlm.nih.gov/pubmed/16005967?dopt=Abstract Paper] [http://dbtbs.hgc.jp/ DBTBS] || J || 81bp | | 2.Promoter - σB || PgsiB || [http://openwetware.org/wiki/Image:PgsiB.doc PgsiB] <br> [http://openwetware.org/wiki/Image:PgsiB_Biobrick.doc Biobrick] || [http://www.ncbi.nlm.nih.gov/pubmed/16005967?dopt=Abstract Paper] [http://dbtbs.hgc.jp/ DBTBS] || J || 81bp | ||
|- | |- | ||
| 3.Promoter - ComK upregulated || PcomC || [http://openwetware.org/wiki/Image:PcomC.doc PcomC] <br> [http://openwetware.org/wiki/Image:PcomC_Biobrick.doc Biobrick] || [http://dbtbs.hgc.jp/ DBTBS] || C || | | 3.Promoter - ComK upregulated || PcomC || [http://openwetware.org/wiki/Image:PcomC.doc PcomC] <br> [http://openwetware.org/wiki/Image:PcomC_Biobrick.doc Biobrick] || [http://dbtbs.hgc.jp/ DBTBS] || C || 94bp (137bp Biobrick) | ||
|- | |- | ||
|4.Promoter - Constitutuive || P43 || [http://openwetware.org/wiki/Image:P43.doc P43] <br> [http://openwetware.org/wiki/Image:P43_Biobrick.doc Biobrick] || [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1169017 Paper] || C || 609bp (with RBS) | |4.Promoter - Constitutuive || P43 || [http://openwetware.org/wiki/Image:P43.doc P43] <br> [http://openwetware.org/wiki/Image:P43_Biobrick.doc Biobrick] || [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1169017 Paper] || C || 609bp (with RBS) | ||
Revision as of 11:37, 29 July 2008
Hello Team Sequence, Thought we could use this page to load up all the information. If we could load up the sequence of the parts here, probably load the pure sequence then one with the restriction sites on the end. Here are some useful websites about the ends:
- http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix
- http://partsregistry.org/Assembly:RBS-CDS_issues
First thing tomorrow we need to get a group together (preferably with Kirsten) and decide about whether to use stand-alone RBSs or use Promoter-RBS or RBS-gene combinations
| Part Class | Part Name | Sequence File | Papers | Checked | Biobrick Length (bp) |
| Promoter | |||||
| 1.Promoter - Light | Pctc | Pctc Biobrick |
Paper DBTBS | J,QQ | 99 |
| 2.Promoter - σB | PgsiB | PgsiB Biobrick |
Paper DBTBS | J | 81bp |
| 3.Promoter - ComK upregulated | PcomC | PcomC Biobrick |
DBTBS | C | 94bp (137bp Biobrick) |
| 4.Promoter - Constitutuive | P43 | P43 Biobrick |
Paper | C | 609bp (with RBS) |
| 5.Promoter - Inducible | Phyper-spank | Ph-s Biobrick |
Dr. Angelika Grundling (Imperial College London), Dr. Jan-Willem Veening (Newcastle University), Paper1, Paper2, Paper3, NEBcutter2 | C | 102bp (145bp Biobrick) |
| 6.Promoter - Inducible | Pxyl | Pxyl Biobrick |
Dr. Jan-Willem Veening (Newcastle University), DBTBS, Paper1, Paper2 | C | 40-44bp (83-87bp Biobrick) |
| 7.Promoter - Contitutive (high) | Pveg | Pveg Biobrick |
Dr. Jan-Willem Veening (Newcastle University), DBTBS | C | 44bp (87bp Biobrick) |
| RBS | |||||
| 1.RBS - Stong | RBS-gsiB | RBS-gsiB Biobrick |
Paper DBTBS | 27bp (54bp biobrick, 343bp with promoter) | |
| 2.RBS - Strong | RBS-spoVG | RBS-spoVG Biobrick |
Paper (reference only) DBTBS | C | 37bp (68bp Biobrick) |
| 3.RBS - Strong | RBS-PBS | RBS-PBS Biobrick |
Dr. Jan-Willem Veening (Newcastle University), Paper | Issues - C | |
| Gene | |||||
| 1.Gene | epsE | Protein Biobrick |
Page 9, Online Supplementary Material for "A Molecular Clutch Disables Flagella in the Bacillus subtilis Biofilm" | QQ, J | 878 |
| 2.Gene | ytvA | Protein Biobrick |
Partial sequence (LOV domain) | QQ, J | 786 |
| 3.Gene | sinR | Protein Biobrick |
- | QQ, J | 377 |
| 4.Biomaterial | Ep24-20-20 | - | |||
| 5.Biomaterial | EAK16-II | Biobrick | Paper | ||
| 6.Signal Peptide | SacB | - | |||
| 7.Signal Peptide | LipA | - | |||
| 8.Signal Peptide | Epr | - | |||
| Terminator | |||||
| 1.Terminator | TermI-VI Consensus of 6 terminators |
6NSCT Protein Biobrick |
Search for additional replication terminators in the Bacillus subtilis chromosome | 30bp |
