IGEM:IMPERIAL/2009

From OpenWetWare
Jump to navigationJump to search
iGEM 2009 - Imperial College London Team

Work in progress ...

ToDo & Deadlines

<html><center><iframe src="http://www.google.com/calendar/embed?mode=WEEK&amp;showTitle=0&amp;showCalendars=0&amp;height=600&amp;wkst=2&amp;bgcolor=%23ffffff&amp;src=imperial.igem09%40googlemail.com&amp;color=%23A32929&amp;ctz=Europe%2FLondon" style=" border-width:0 " width="800" height="600" frameborder="0" scrolling="no"></iframe></center></html>

Useful Links


Wiki Updates

List of abbreviations:
N
This edit created a new page (also see list of new pages)
m
This is a minor edit
b
This edit was performed by a bot
(±123)
The page size changed by this number of bytes

18 April 2024

     15:01  Pan:Who we are diffhist +14 Taopan talk contribs
     15:00  Pan:Methods‎‎ 2 changes history +456 [Taopan‎ (2×)]
     
15:00 (cur | prev) +2 Taopan talk contribs
     
14:59 (cur | prev) +454 Taopan talk contribs
     14:56  Pan:Publications‎‎ 2 changes history +396 [Taopan‎ (2×)]
     
14:56 (cur | prev) +74 Taopan talk contribs
     
14:54 (cur | prev) +322 Taopan talk contribs
     13:03  BioMicroCenter:Pricing diffhist +166 Challee talk contribs
     12:58  BioMicroCenter:Singular Sequencing‎‎ 2 changes history +124 [Challee‎ (2×)]
     
12:58 (cur | prev) +14 Challee talk contribs (→‎Things to Consider)
     
12:57 (cur | prev) +110 Challee talk contribs
     12:12  BioMicroCenter:Tecan Freedom Evo‎‎ 7 changes history +1,746 [Noelani Kamelamela‎ (7×)]
     
12:12 (cur | prev) +4 Noelani Kamelamela talk contribs
     
12:12 (cur | prev) +3 Noelani Kamelamela talk contribs
     
10:13 (cur | prev) +7 Noelani Kamelamela talk contribs (→‎verrity Chemagic 360)
     
10:08 (cur | prev) −42 Noelani Kamelamela talk contribs (→‎verrity Chemagic 360)
     
10:08 (cur | prev) +86 Noelani Kamelamela talk contribs (→‎verrity Chemagic 360)
     
09:34 (cur | prev) +23 Noelani Kamelamela talk contribs (→‎verrity Chemagic 360)
     
09:32 (cur | prev) +1,665 Noelani Kamelamela talk contribs
     11:42  3D Cell Culture - McLean Taggart, Emma Villares, Maximillian Marek, Scott LeBlanc, Adam Lyons and Jacob Belden diffhist −3 Sarah L. Perry talk contribs
     09:35  BioMicroCenter‎‎ 2 changes history +92 [Noelani Kamelamela‎ (2×)]
     
09:35 (cur | prev) +60 Noelani Kamelamela talk contribs
     
09:20 (cur | prev) +32 Noelani Kamelamela talk contribs
     09:32 Upload log Noelani Kamelamela talk contribs uploaded File:Chemagic360.jpg(from manual)

17 April 2024

     15:34  BioMicroCenter:Element Sequencing‎‎ 3 changes history +295 [Challee‎ (3×)]
     
15:34 (cur | prev) +195 Challee talk contribs
     
14:22 (cur | prev) +100 Challee talk contribs
     
14:07 (cur | prev) 0 Challee talk contribs
     13:10  BioMicroCenter:SingleCell diffhist +30 Noelani Kamelamela talk contribs (→‎10X CHROMIUM X)
     12:43  BioMicroCenter diffhist −15 Noelani Kamelamela talk contribs

16 April 2024

N    19:59  Nanoimprint Lithography (NIL) - Carter Paul‎‎ 10 changes history +7,205 [CarterPaul‎ (10×)]
     
19:59 (cur | prev) +769 CarterPaul talk contribs (→‎Thermal NIL Process)
     
19:53 (cur | prev) 0 CarterPaul talk contribs (→‎Thermal NIL Process)
     
19:53 (cur | prev) 0 CarterPaul talk contribs (→‎Thermal NIL Process)
     
19:52 (cur | prev) +1 CarterPaul talk contribs (→‎Thermal NIL Process)
     
19:50 (cur | prev) +202 CarterPaul talk contribs (→‎Thermal NIL Process)
     
19:17 (cur | prev) −20 CarterPaul talk contribs (→‎References)
     
19:17 (cur | prev) −1 CarterPaul talk contribs
     
19:11 (cur | prev) +4,278 CarterPaul talk contribs
     
18:53 (cur | prev) +1,891 CarterPaul talk contribs
N    
18:42 (cur | prev) +85 CarterPaul talk contribs (Created page with "{{Template:CHEM-ENG590E}} =Motivation= =Introduction to NIL= =Thermal NIL Process=")
     19:40 Upload log CarterPaul talk contribs uploaded File:NIL1.png
N    18:40  3D Cell Culture - McLean Taggart, Emma Villares, Maximillian Marek, Scott LeBlanc, Adam Lyons and Jacob Belden diffhist +24,060 CarterPaul talk contribs (Created page with "{{Template:CHEM-ENG590E}} ==Introduction== While most microfluidic devices incorporate a 2D cell culture design, in which a single layer of cells is grown on the bottom of a device, these systems suffer from poor <i>in vivo</i> mimicry, as, in the human body, most cells grow in all directions.<sup>https://doi.org/10.5114/aoms.2016.63743 1</sup> To address this limitation, 3D cell culture devices have been developed - in w...")
     18:38  CHEM-ENG590E:Wiki Textbook‎‎ 2 changes history +63 [CarterPaul‎ (2×)]
     
18:38 (cur | prev) +50 CarterPaul talk contribs (→‎Chapter 1 - Microfabrication)
     
18:37 (cur | prev) +13 CarterPaul talk contribs
     18:36  3D Cell Culture - McLean Taggart, Emma Villares, Maximillian Marek, Scott LeBlanc, and Adam Lyons diffhist +5,343 CarterPaul talk contribs (Added a Technique and applications section)
     10:20  Yarn Microfluidics - Roger Dirth‎‎ 11 changes history +406 [Rcostello‎ (11×)]
     
10:20 (cur | prev) +41 Rcostello talk contribs (→‎Applications)
     
10:19 (cur | prev) +36 Rcostello talk contribs (→‎Applications)
     
10:18 (cur | prev) +36 Rcostello talk contribs (→‎Introduction)
     
10:17 (cur | prev) +38 Rcostello talk contribs (→‎Fabrication)
     
10:17 (cur | prev) +38 Rcostello talk contribs (→‎Washburn Equation)
     
10:16 (cur | prev) +38 Rcostello talk contribs (→‎Wicking Rate)
     
10:16 (cur | prev) +37 Rcostello talk contribs (→‎Introduction)
     
10:15 (cur | prev) +36 Rcostello talk contribs (→‎Wicking Rate)
     
10:14 (cur | prev) +36 Rcostello talk contribs (→‎Fabrication)
     
10:14 (cur | prev) +34 Rcostello talk contribs (→‎Applications)
     
10:14 (cur | prev) +36 Rcostello talk contribs (→‎Introduction)


Synthetic Biology @ Imperial

iGEM resources

Advisor Contributions

Schumann lab from Uni. Beyreuth, DE have done some interesting work on using spores to direct antigens to the gut - to act as vaccines. Sporulation guys might also be interested in this paper, describing the B. sub coat protein (and how it's hilariously complex but not all required). Oh, and subtilis spores will germinate in the gut (probably), justifying using the killswitch!

Killswitch guys, I think perhaps looking into recombinases as opposed to restriction enzymes would be useful as they won't act on host DNA. Xer and Dif sites will recombine with themselves in presence of the required enzyme, excising any genes between them - you could flank genes with them, then express the enzyme to chop your construct up. Sites are required to be within ≈5kb of each other, I think, so random ones on host DNA shouldn't be affected. It might take a while to work so look into the time; could be useful as a fallback, anyway.

Biobrick images you can use if you need (advisors like named/labelled circuit diagrams!):

~ Tom Adie 15:44, 20 July 2009 (EDT)

<html>

<!-- Start of StatCounter Code --> <script type="text/javascript"> var sc_project=4444613; var sc_invisible=1; var sc_partition=55; var sc_click_stat=1; var sc_security="fb1ae6cb"; </script>

<script type="text/javascript" src="http://www.statcounter.com/counter/counter.js"></script><noscript><div class="statcounter"><a title="blogger counter" href="http://www.statcounter.com/blogger/" target="_blank"><img class="statcounter" src="http://c.statcounter.com/4444613/0/fb1ae6cb/1/" alt="blogger counter" ></a></div></noscript> <!-- End of StatCounter Code --> </html>