IGEM:IMPERIAL/2009
iGEM 2009 - Imperial College London Team
The E.ncapsulator
ToDo & Deadlines
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Contents
- Assay Protocols and Cloning Strategies
- BioBrick Designs
- Schematics and Parts
- Primers for PCR
- Specifications by Module
- Past Presentations
- Brainstorming
Useful Links
Team Roles
Change your role when applicable
Charles - Timer
Dave - Assays, cloning strategy/constructs
Dineka - BioBricks
James - Wet lab, Testing Constructs
Kun - Assays, constructs
Nuri - Modelling
Royah - Wet lab, In-Fusion cloning/SLIC, protocols for testing promoters
Tianyi - Modelling
Wiki Updates
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- This edit created a new page (also see list of new pages)
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22 April 2024
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19:28 | "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello 4 changes history +1 [Rcostello (4×)] | |||
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19:28 (cur | prev) −2 Rcostello talk contribs (→Nanowires) | ||||
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19:26 (cur | prev) 0 Rcostello talk contribs (→Biology-Inspired Solution) | ||||
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15:03 (cur | prev) +2 Rcostello talk contribs (→At the Microscale) | ||||
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15:02 (cur | prev) +1 Rcostello talk contribs (→Overview) | ||||
| 09:24 | CHEM-ENG590E:Wiki Textbook diffhist +16 Rcostello talk contribs (→Chapter 15 - Other Topics) | ||||
| 09:24 | Move log Rcostello talk contribs moved page "Pick and Place" Assembly of Parts Using PDMS - Amy Lim to "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello | ||||
| 08:59 | "Pick and Place" Assembly of Parts Using PDMS - Amy Lim diffhist −2,792 Rcostello talk contribs (→"Pick and Place" for Microfluidics) | ||||
21 April 2024
| 22:55 | Lee:JC diffhist +24 Wooin Lee talk contribs (→2024) | ||||
| 17:42 | Ernesto-Perez-Rueda:Publications diffhist +55 Ernesto Perez-Rueda talk contribs | ||||
19 April 2024
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21:58 | Hu 2 changes history +58 [Hugangqing (2×)] | |||
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18 April 2024
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15:00 | Pan:Methods 2 changes history +456 [Taopan (2×)] | |||
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14:56 | Pan:Publications 2 changes history +396 [Taopan (2×)] | |||
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| 13:03 | BioMicroCenter:Pricing diffhist +166 Challee talk contribs | ||||
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12:58 | BioMicroCenter:Singular Sequencing 2 changes history +124 [Challee (2×)] | |||
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12:58 (cur | prev) +14 Challee talk contribs (→Things to Consider) | ||||
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12:57 (cur | prev) +110 Challee talk contribs | ||||
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12:12 | BioMicroCenter:Tecan Freedom Evo 7 changes history +1,746 [Noelani Kamelamela (7×)] | |||
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10:13 (cur | prev) +7 Noelani Kamelamela talk contribs (→verrity Chemagic 360) | ||||
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10:08 (cur | prev) −42 Noelani Kamelamela talk contribs (→verrity Chemagic 360) | ||||
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09:34 (cur | prev) +23 Noelani Kamelamela talk contribs (→verrity Chemagic 360) | ||||
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09:32 (cur | prev) +1,665 Noelani Kamelamela talk contribs | ||||
| 11:42 | 3D Cell Culture - McLean Taggart, Emma Villares, Maximillian Marek, Scott LeBlanc, Adam Lyons and Jacob Belden diffhist −3 Sarah L. Perry talk contribs | ||||
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09:35 | BioMicroCenter 2 changes history +92 [Noelani Kamelamela (2×)] | |||
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| 09:32 | Upload log Noelani Kamelamela talk contribs uploaded File:Chemagic360.jpg (from manual) | ||||
17 April 2024
Synthetic Biology @ Imperial
- Institute of Systems and Synthetic Biology
- Center of Synthetic Biology (press release)
- Synthetic Biology Course @ Imperial
iGEM resources
Advisor Contributions
Schumann lab from Uni. Beyreuth, DE have done some interesting work on using spores to direct antigens to the gut - to act as vaccines. Sporulation guys might also be interested in this paper, describing the B. sub coat protein (and how it's hilariously complex but not all required). Oh, and subtilis spores will germinate in the gut (probably), justifying using the killswitch!
Killswitch guys, I think perhaps looking into recombinases as opposed to restriction enzymes would be useful as they won't act on host DNA. Xer and Dif sites will recombine with themselves in presence of the required enzyme, excising any genes between them - you could flank genes with them, then express the enzyme to chop your construct up. Sites are required to be within ≈5kb of each other, I think, so random ones on host DNA shouldn't be affected. It might take a while to work so look into the time; could be useful as a fallback, anyway.
Biobrick images you can use if you need (advisors like named/labelled circuit diagrams!): 
~ Tom Adie 15:44, 20 July 2009 (EDT)
Killing guys, things to keep in mind for the restriction enzymes...
1. Restriction enzyme:
- Cuts at short sequences; makes it easier to insert (see next point) and will cut in the genome and plasmid more often by chance
- Restriction sites be inserted by codon changes; GeneArt optimise constructs to remove restriction sites all the time, so putting them in should be OK
- Is not native; if it's expressed somehow by the cell, it'll suicide on its own
2. Regulation of expression:
- Needs to be near 100% off when off; even a low level of expression will destroy your cells long before any product is produced
- You may want to look for a less-efficient enzyme so small leakage wouldn't be that bad; or an enzyme that is degraded relatively quickly
- Bistable switch with no leakage might be good; or flippase regulation! My pet idea for the bioremediation project last year =P
You can also insert sites after the transcription terminators in all your genes to ensure full destruction of the construct, and maybe add your sites into normal bricks (terminators etc.) and put them up as variants.
~ Tom Adie 11:41, 23 July 2009 (EDT)
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