IGEM:IMPERIAL/2009/Assays Protocols/Cloning Strategy M1&T: Difference between revisions
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== Module 1 Cloning Strategy == | == Module 1 Cloning Strategy == | ||
[[Image:II09_clonstratm1. | [[Image:II09_clonstratm1.jpg]] | ||
* Ligate PAH/ Cellulase gene sequence (G1) from GeneArt with the arabinose-inducible promoter from the iGEM starter kit. | * Ligate PAH/ Cellulase gene sequence (G1) from GeneArt with the arabinose-inducible promoter from the iGEM starter kit. | ||
Revision as of 06:22, 29 July 2009
Module 1 Cloning Strategy
- Ligate PAH/ Cellulase gene sequence (G1) from GeneArt with the arabinose-inducible promoter from the iGEM starter kit.
- Test for successful ligation using either:
- Cellulose/ PAH assay as defined on OWW
- Sample culture, and use GFP reporter to test for activity.
- If successful, remove terminators from G1 gene sequence using suitable restriction enzymes.
- Ligate sequence to LacI (BBa_C0012) from the iGEM starter kit.
