IGEM:IMPERIAL/2009/Assays Protocols/M3 Assays: Difference between revisions
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==Assays for Cell Death Strategy== | |||
<i>Basic genetic circuit for the restriction enzyme testing construct: </i> | |||
[[Image:II09_Killtestm3.png]] | [[Image:II09_Killtestm3.png]] | ||
Here, there are 3 possibilities for the expression of the GFP, and these are shown below: | |||
[[Image:II09_m3_restenz.png]] | [[Image:II09_m3_restenz.png]] | ||
i) Non-functional restriction enzyme co-expressed with the GFP. | |||
ii) Basal levels of GFP expression suggesting that the promoter is not functioning properly. | |||
iii) Initial increase in flourescence, followed by a plateauing of the flourescence, as the restriction enzymes destroy the genetic material within the cell. | |||
Revision as of 07:19, 3 August 2009
Assays for Cell Death Strategy
Basic genetic circuit for the restriction enzyme testing construct:
Here, there are 3 possibilities for the expression of the GFP, and these are shown below:
i) Non-functional restriction enzyme co-expressed with the GFP. ii) Basal levels of GFP expression suggesting that the promoter is not functioning properly. iii) Initial increase in flourescence, followed by a plateauing of the flourescence, as the restriction enzymes destroy the genetic material within the cell.
