IGEM:IMPERIAL/2009/Assays Protocols/Shopping List

Shopping List

• Food grade sodium acetate : Sigma Purchasing Page
  

Module 1

Sigma Products for Cellulase Assay:

No. S-8625 = Sodium Acetate, Trihydrate :Sigma Purchasing Page

No. S-3504 = Sigmacell Solution (Sigmacell) Sigma Purchasing Page

No. 16-10 = Glucose (HK) Sigma Purchasing Page

Sigma Products for PAH Assay:

Trizma Base : Sigma Prod. No. T-1503

L-Phenylalanine : Sigma Prod. No. P-2126

Catalase : Sigma Stock No. C-100

DL-Dithiothreitol : Sigma Prod. No. D-0632

DL-6-Methyl-5,6,7,8-Tetrahydropterine Dihydrochloride : Sigma Prod. No. M-4758

Trichloroacetic Acid, 6.1 N Solution : Sigma Stock No.490-10

Nitric Acid : Aldrich Stock No. 25811-3

Sodium Nitrite : Sigma Prod. No. S-2252

1-Nitroso-2-Naphthol : Sigma Prod. No. N-3765

Sodium Hydroxide Solution : 1.0 N, Sigma Stock No. 930-65

L-Tyrosine Free Base : Sigma Prod. No. T-3754

Module 2

Equipment required for Cell Colanic Acid Assay
PCV Centrifuge Tubes :Purchasing Page

Trehalose 6-Phosphate Synthase Assay (OtsA)
Reagents:
HEPES/ KOH (pH 6.8)
MgCl2 ; Sigma prod no. m8266: Sigma Order Page
Glucose-6-Phosphate; Sigma Prod no. G7772 : Sigma Order Page
UDPG
Bovine albumin; Sigma Prod. No. A8531 : Sigma Order Page
Phosphoenolpyruvate ; Sigma Prod No. 860077 : Sigma Order Page
NADH
Pyruvate kinase Sigma Order Page
Lactate dehydrogenase; Prod. No. 59747 : Sigma Order Page

Trehalose Assay
Standard Trehalose Test Kit : Megazyme Order Page

Other required Reagents:
Sodium Hydroxide
Alkaline borohydride (10 mg/mL sodium borohydride in 50 mM sodium hydroxide); Sigma Prod. No. S9125 : Sodium Borohydride Sigma Order Page
Acetic Acid

Module 3

Cell Death Test
Cell Membrane Staining Kit Biotium Purchasing Page