IGEM:IMPERIAL/2009/Encapsulation/Biobricks: Difference between revisions
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<td>Constitutive promoter</td> | <td>Constitutive promoter</td> | ||
<td>BBa_J23114</td> | <td>BBa_J23114</td> | ||
<td> | <td>KP1 2009</td> | ||
<td>20I</td> | <td>20I</td> | ||
<td>BBa_J61002</td> | <td>BBa_J61002</td> | ||
Revision as of 14:24, 6 August 2009
Biobricks
Module 1
Module 2
Module 3
Module 3 (provisional) biobricks
Module Timer
Biobricks from the registry
Table 1. Biobricks we need from the registry
| Module | Part | BB # | Kit | Well # | Plasmid | Resistance | Copy # | Link |
| 1 | LacIQ promoter | BBa_K091111 | Shipment: 00352 ID:941 Davidson | 2 | pSB1A2 | A | 100-300 | LacIQ promoter |
| 1,2,3 | RBS | BBa_B0034 | KP1 2009 | 2M | pSB1A2 | A | 100-300 | RBS |
| 1,2,3 | Double terminator | BBa_B0015 | KP1 2009 | 23L | pSB1AK3 | AK | 100-300 | Double terminator |
| 1,2,3 | GFP generator | BBa_E0240 | KP1 2009 | 12M | pSB1A2 | A | 100-300 | GFP generator |
| 1,2,3 | Variable input (testing) | BBa_F2620 | KP2 2009 | 6E | pSB1A2 | A | 100-300 | Variable input |
| 3 | Constitutive promoter | BBa_J23114 | KP1 2009 | 20I | BBa_J61002 | A | 15-20 (based on pBR322 ORI) | Constitutive promoter |
| 3 | Constitutive promoter | BBa_J23103 | KP2 2009 | 18I | BBa_J61002 | A | 15-20 (based on pBR322 ORI) | Constitutive promoter |
| 3 | Promoter (lambda cI regulated) | BBa_R0051 | KP1 2009 | 6E | pSB1A2 | A | 100-300 | Promoter (lambda cI regulated) |
| 3 | cI QPI with high promoter (heat sensitive) | BBa K098995 | KP3 2009 | 1E | pSB1A2 | A | 100-300 | cI QPI with high promoter |
| 3 | pBAD Promoter | BBa_I0500 | KP1 2009 | 14N | pSB2K3 | K | Variable – IPTG results in high copy # (++100 per cell) | pBAD Promoter |
| Timer | Promoter(luxR & HSL regulated-lux pR) | BBa_R0062 | KP1 2009 | 6O | pSB1A2 | A | 100-300 | lux pR |
| Timer | Assembly | BBa_S03119 | KP1 2009 | 10O | pSB1A2 | A | 100-300 | Assembly |
| Timer | LacI-LuxI | BBa_K084014 | KP2 2009 | 2B | pSB1A2 | A | 100-300 | LacI-LuxI |
| Timer | LacI-LasI | BBa_K084007 | KP3 2009 | 12E | pSB1AK3 | AK | 100-300 | LacI-LasI |
| Timer | Lac repressible RhlI generator | BBa_K084008 | KP3 2009 | 12C | pSB1AK3 | AK | 100-300 | Lac repressible RhlI generator |
Biobricks
Under construction
| Gene | NCBI Ref | Nucleotide accession# | Organism | Xbal & SpeI sites | Start? | Stop? | ORF | Sequence | References |
| PAH | 5053 | PAH # | Homo Sapiens | 1 and 0 | Yes | Yes | 1359bp (+1) | Add | PAH |
| Cellulase | 4810415 | Cellulase # | Clostridium thermocellum | 0 and 0 | Yes | Yes | 2445bp (+1) | Add | Add |
| RcsB | 947441 | RcsB # | K12 MG1655 | 0 and 0 | Yes | Yes | 651bp (+1) | Add | Add |
| B3023 | 945805 | B3023 # | K12 MG1655 | 0 and 0 | Yes | Yes | 483bp (+1) | Add | Add |
| WaaL | 948148 | WaaL # | K12 MG1655 | 0 and 0 | Yes | Yes | 1260bp (+1) | Add | Add |
| OtsA | 946405 | OtsA # | K12 MG1655 | 0 and 0 | Yes | Yes | 1425bp (+1) | Add | Add |
| OtsB | 946406 | OtsB # | K12 MG1655 | 0 and 0 | Yes | Yes | 612bp (+1) | Add | Add |
| Dam | 947893 | Dam # | K12 MG1655 | 0 and 0 | Yes | Yes | 837bp (+1) | Add | Add |
| TaqI | 6941926 | TaqI # | Thermodesulfovibrio yellowstonii DSM 11347 | 0 and 0 | Yes | Yes | 777bp (+1) | Add | Add |
| Dpn2 | 6029545 | Dpn2 # | Strepococcus pneumoniae Hungary19A-6 | 0 and 0 | Yes | Yes | 867bp (+1) | Add | Add |
Genes and biobrick compatibility
|
PAH | Cellulase | rcsB | B3023 | waaL | OtsA | OtsB |
| EcoRI | No | No | No | No | No | No | No |
| SpeI | No | No | No | No | No | No | No |
| XbaI | Yes | No | No | No | No | No | No |
| PstI | Yes | No | Yes | Yes | No | No | No |
| NotI | No | No | No | Yes | No | No | No |
| PvuII | Yes (3) | Yes | No | No | No | Yes | No |
| XhoI | Yes | No | No | No | No | No | No |
| AvrII | No | No | No | No | Yes | No | No |
| NheI | No | No | No | No | Yes | No | No |
| SapI | No | No | No | No | No | No | No |
| Sequence modified? | Yes | Yes |
